Immunoassay Method for the Determination of Immunoglobulin G Using Bacterial Magnetic Particles

Noriyuki Nakamura, Kohji Hashimoto, Tadashi Matsunaga

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Abstract

We have developed a novel Immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.

Original languageEnglish
Pages (from-to)268-272
Number of pages5
JournalAnalytical Chemistry
Volume63
Issue number3
DOIs
Publication statusPublished - 1991 Feb 1
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry

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