Immunoassay Method for the Determination of Immunoglobulin G Using Bacterial Magnetic Particles

Noriyuki Nakamura, Kohji Hashimoto, Tadashi Matsunaga

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

We have developed a novel Immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.

Original languageEnglish
Pages (from-to)268-272
Number of pages5
JournalAnalytical Chemistry
Volume63
Issue number3
DOIs
Publication statusPublished - 1991 Feb 1
Externally publishedYes

Fingerprint

Immunoglobulin G
Fluorescence
Fluorescein
Bacterial Antibodies
Spectrophotometers
Gelatin
Quenching
Agglomeration
Magnetic fields
isothiocyanic acid

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Immunoassay Method for the Determination of Immunoglobulin G Using Bacterial Magnetic Particles. / Nakamura, Noriyuki; Hashimoto, Kohji; Matsunaga, Tadashi.

In: Analytical Chemistry, Vol. 63, No. 3, 01.02.1991, p. 268-272.

Research output: Contribution to journalArticle

@article{e35152903970468d892124788b71d9a0,
title = "Immunoassay Method for the Determination of Immunoglobulin G Using Bacterial Magnetic Particles",
abstract = "We have developed a novel Immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.",
author = "Noriyuki Nakamura and Kohji Hashimoto and Tadashi Matsunaga",
year = "1991",
month = "2",
day = "1",
doi = "10.1021/ac00003a015",
language = "English",
volume = "63",
pages = "268--272",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Immunoassay Method for the Determination of Immunoglobulin G Using Bacterial Magnetic Particles

AU - Nakamura, Noriyuki

AU - Hashimoto, Kohji

AU - Matsunaga, Tadashi

PY - 1991/2/1

Y1 - 1991/2/1

N2 - We have developed a novel Immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.

AB - We have developed a novel Immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.

UR - http://www.scopus.com/inward/record.url?scp=0026102793&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026102793&partnerID=8YFLogxK

U2 - 10.1021/ac00003a015

DO - 10.1021/ac00003a015

M3 - Article

VL - 63

SP - 268

EP - 272

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 3

ER -