Immunochromatographic assay of cadmium levels in oysters

Kosuke Nishi, In Hae Kim, Takaaki Itai, Takuya Sugahara, Haruko Takeyama, Hideo Ohkawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Oysters are one of foodstuffs containing a relatively high amount of cadmium. Here we report on establishment of an immunochromatographic assay (ICA) method of cadmium levels in oysters. Cadmium was extracted with 0.l mol L -1 HCl from oysters and cleaned up from other metals by the use of an anion-exchange column. The behavior of five metals Mn, Fe, Cu, Zn, and Cd was monitored at each step of extraction and clean-up procedure for the ICA method in an inductively coupled plasma-mass spectrometry (ICP-MS) analysis. The results revealed that a simple extraction method with the HCl solution was efficient enough to extract almost all of cadmium from oysters. Clean-up with an anion-exchange column presented almost no loss of cadmium adsorbed on the column and an efficient removal of metals other than cadmium. When a spiked recovery test was performed in the ICA method, the recovery ranged from 98% to 112% with relative standard deviations between 5.9% and 9.2%. The measured values of cadmium in various oyster samples in the ICA method were favorably correlated with those in ICP-MS analysis (r2=0.97). Overall results indicate that the ICA method established in the present study is an adequate and reliable detection method for cadmium levels in oysters.

Original languageEnglish
Pages (from-to)262-266
Number of pages5
JournalTalanta
Volume97
DOIs
Publication statusPublished - 2012 Aug 15

Keywords

  • Antibody
  • Cadmium
  • Heavy metal
  • Immnochromatography
  • Oyster

ASJC Scopus subject areas

  • Analytical Chemistry

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  • Cite this

    Nishi, K., Kim, I. H., Itai, T., Sugahara, T., Takeyama, H., & Ohkawa, H. (2012). Immunochromatographic assay of cadmium levels in oysters. Talanta, 97, 262-266. https://doi.org/10.1016/j.talanta.2012.04.028