Immunochromatographic detection of MPB64 secreted from active BCG by heating: Toward same-day diagnosis of tuberculosis

Kazunari Nakaishi, Satoshi Watabe, Toshiyuki Kitagawa, Etsuro Ito

Research output: Contribution to journalArticle

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Abstract

We propose a new detection method for a tuberculosis-specific protein, MPB64, obtained from active bacillus Calmette-Guérin (BCG) by heating. When BCG was included in solution at a concentration >2.75 × 10 4 CFU/ml, our method for collecting MPB64 through heating active BCG combined with an immunochromatographic assay detected active bacilli within 2.5 h. By contrast, a culture test, which is the gold standard for tuberculosis diagnosis, does not provide results for between 1 week and 2 months. The rapid tests based on PCR have some drawbacks, for example they detect DNA from both active and latent (or even dead) tubercle bacilli. Therefore, our method may pave the way toward detecting only active tubercle bacilli at a reasonable cost and providing same-day diagnosis.

Original languageEnglish
Pages (from-to)240-242
Number of pages3
JournalBioTechniques
Volume66
Issue number5
DOIs
Publication statusPublished - 2019 Jan 1

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Keywords

  • Immunochromatography
  • MPB64
  • Mycobacterium tuberculosis
  • Same-day diagnosis

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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