Immunohistochemical localization of GnRH and RFamide-related peptide-3 in the ovaries of mice during the estrous cycle

Padmasana Singh, Amitabh Krishna, Rajagopala Sridaran, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Gonadotropin releasing hormone (GnRH) has now been suggested as an important intraovarian regulatory factor. Gonadotropin inhibitory hormone (GnIH) a hypothalamic dodecapeptide, acts opposite to GnRH. GnRH, GnIH and their receptors have been demonstrated in the gonads. In order to find out the physiological significance of these neuropeptides in the ovary, we aim to investigate changes in the abundance of GnRH I and GnIH in the ovary of mice during estrous cycle. The present study investigated the changes in GnRH I, GnRH I-receptor and RFRP-3 protein expression in the ovary of mice during estrous cycle by immunohistochemistry and immunoblot analysis. The immunoreactivity of GnRH I and its receptor and RFRP-3 were mainly localized in the granulosa cells of the healthy and antral follicles during proestrus and estrus and in the luteal cells during diestrus 1 and 2 phases. The relative abundance of immunoreactivity of GnRH I, GnRH I-receptor and RFRP-3 undergo significant variation during proestrus and thus may be responsible for selection of follicle for growth and atresia. A significant increase in the concentration of RFRP-3 during late diestrus 2 coincided with the decline in corpus luteum activity and initiation of follicular growth and selection. In general, immunolocalization of GnRH I, GnRH I-receptor and RFRP-3 were found in close vicinity suggesting functional interaction between these peptides. It is thus, hypothesized that interaction between GnRH I-RFRP-3 neuropeptides may be involved in the regulation of follicular development and atresia.

    Original languageEnglish
    Pages (from-to)371-381
    Number of pages11
    JournalJournal of Molecular Histology
    Volume42
    Issue number5
    DOIs
    Publication statusPublished - 2011 Oct

    Fingerprint

    Estrous Cycle
    Gonadotropin-Releasing Hormone
    Ovary
    LHRH Receptors
    Gonadotropins
    Diestrus
    Proestrus
    Neuropeptides
    Follicular Atresia
    Hormones
    Hypothalamic Hormones
    Luteal Cells
    RFamide peptide
    Granulosa Cells
    Corpus Luteum
    Estrus
    Gonads
    Growth
    Immunohistochemistry
    Peptides

    Keywords

    • Estrous cycle
    • GnRH I
    • GnRH I-receptor
    • Mice
    • Ovary
    • RFRP-3

    ASJC Scopus subject areas

    • Cell Biology
    • Histology
    • Physiology

    Cite this

    Immunohistochemical localization of GnRH and RFamide-related peptide-3 in the ovaries of mice during the estrous cycle. / Singh, Padmasana; Krishna, Amitabh; Sridaran, Rajagopala; Tsutsui, Kazuyoshi.

    In: Journal of Molecular Histology, Vol. 42, No. 5, 10.2011, p. 371-381.

    Research output: Contribution to journalArticle

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