Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin

Hiroko Ikeshima-Kataoka, Yoichiro Abe, Takaya Abe, Masato Yasui

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    During injury to the central nervous system (CNS), astrocytes and microglia proliferate and migrate around the lesion sites. Recently, it has been reported that one of the water channels, aquaporin-4 (AQP4) is seemed to have a role in astroglial migration and glial scar formation caused by brain injury, although its molecular mechanism is largely unknown. In the present study, we examined the expression profiles in wild-type (WT) and AQP4-deficient (AQP4/KO) mice after a stab wound to the cerebral cortex. Three days after the stab wound, AQP4 expression was observed in activated microglia around the lesion site as well as in astrocytes. A microarray analysis revealed that 444 genes around the lesion site were upregulated 3. days after the wounding in WT mice. Surprisingly, most of these up-regulations were significantly attenuated in AQP4/KO mice. Real-time RT-PCR and immunofluorescence showed that osteopontin (OPN) expression around the lesion site was much lower in AQP4/KO mice than in WT mice. Moreover, the up-regulation of pro-inflammatory cytokines was significantly attenuated in AQP4/KO mice. Taken together, these results suggest that AQP4 plays an important role in immunological function in concert with OPN under pathological conditions in the CNS.

    Original languageEnglish
    Pages (from-to)65-75
    Number of pages11
    JournalMolecular and Cellular Neuroscience
    Volume56
    DOIs
    Publication statusPublished - 2013 Sep

    Fingerprint

    Aquaporin 4
    Osteopontin
    Cytokines
    Brain
    Stab Wounds
    Microglia
    Astrocytes
    Up-Regulation
    Central Nervous System
    Aquaporins
    Microarray Analysis
    Neuroglia
    Cerebral Cortex
    Brain Injuries
    Cicatrix
    Fluorescent Antibody Technique
    Real-Time Polymerase Chain Reaction

    Keywords

    • AQP4
    • Astrocytes
    • Microarray
    • Microglia
    • OPN

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience
    • Cell Biology

    Cite this

    Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin. / Ikeshima-Kataoka, Hiroko; Abe, Yoichiro; Abe, Takaya; Yasui, Masato.

    In: Molecular and Cellular Neuroscience, Vol. 56, 09.2013, p. 65-75.

    Research output: Contribution to journalArticle

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