Immunological Helicobacter pylori urease analyzer based on ion-sensitive field effect transistor

Tetsushi Sekiguchi, Michihiro Nakamura, Mototsugu Kato, Keiko Nishikawa, Kaku Hokari, Toshiro Sugiyama, Masahiro Asaka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Immunological Helicobacter pylori (H. pylori) urease analyzer (HPUA), based on a solid-phase tip coated with a monoclonal antibody toward H. pylori's urease and ion-sensitive field effect transistor (ISFET), was developed. In this system, H. pylori urease adsorbed on the solid-phase tip, after a 15-min immunological reaction with gastric mucus sample solution, was measured with a urease analyzer composed of a flow-through cell for urea solution equipped with the measuring and reference ISFETs. The pH change (ΔpH) of urea solution after 55 s of the enzymatic reaction inside the tip was measured by withdrawing about 1 μl of the urea solution toward the upstream of the tip, where the measuring ISFET is installed. It was confirmed that the present system could detect 0.2 mIU/ml of H. pylori urease. Clinical evaluations of the system were performed for 119 patients using urea breath test (UBT) as a gold standard, resulting in the sensitivity = 33/36 = 92% and specificity = 81/83 = 98%, respectively.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume67
Issue number3
DOIs
Publication statusPublished - 2000 Sep 1
Externally publishedYes

Fingerprint

Ion sensitive field effect transistors
Urease
ureas
Urea
analyzers
field effect transistors
solid phases
ions
mucus
Monoclonal antibodies
antibodies
upstream
Monoclonal Antibodies
evaluation
sensitivity
cells

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

Cite this

Immunological Helicobacter pylori urease analyzer based on ion-sensitive field effect transistor. / Sekiguchi, Tetsushi; Nakamura, Michihiro; Kato, Mototsugu; Nishikawa, Keiko; Hokari, Kaku; Sugiyama, Toshiro; Asaka, Masahiro.

In: Sensors and Actuators, B: Chemical, Vol. 67, No. 3, 01.09.2000, p. 265-269.

Research output: Contribution to journalArticle

Sekiguchi, Tetsushi ; Nakamura, Michihiro ; Kato, Mototsugu ; Nishikawa, Keiko ; Hokari, Kaku ; Sugiyama, Toshiro ; Asaka, Masahiro. / Immunological Helicobacter pylori urease analyzer based on ion-sensitive field effect transistor. In: Sensors and Actuators, B: Chemical. 2000 ; Vol. 67, No. 3. pp. 265-269.
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