Impact of a competitive marathon race on systemic cytokine and neutrophil responses

Katsuhiko Suzuki, Shigeyuki Nakaji, Mutsuo Yamada, Qiang Liu, Shigeyoshi Kurakake, Noriyoshi Okamura, Takashi Kumae, Takashi Umeda, Kazuo Sugawara

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Purpose: To investigate whether cytokines and neutrophils mediate exercise-related pathogenesis, we examined their responses and possible association after exhaustive exercise. Methods: Plasma and urine samples were obtained from 10 male runners before and after a 42.195-km marathon race. Major cytokines and neutrophil activation markers [myeloperoxidase (MPO) and lactoferrin (LTF)] were measured by enzyme-linked immunosorbent assays. Functional modulation of standard neutrophils and monocytes by plasma was determined in vitro on their luminol-dependent chemiluminescence responses. Results: The race induced peripheral neutrophilia accompanied by an increase in band neutrophils and monocytosis. Plasma MPO and LTF concentrations increased significantly by 1.8 and 1.4 times after the race. There was a greater increase in urine concentrations of MPO and LTF, 12.3 and 3.5 times after exercise, respectively, suggesting that neutrophil activation occurred and that renal clearance exceeded the increase in plasma concentrations. Plasma interleukin (IL)-6, IL-8, IL-10, granulocyte colony-stimulating factor (G-CSF), macrophage CSF (M-CSF), and monocyte chemotactic protein 1 (MCP-1) increased significantly after the race, and urine IL-1β, IL-6, G-CSF, M-CSF, and MCP-1 increased significantly. The plasma IL-6 responses correlated with the increases of band neutrophil count (r = 0.860, P < 0.01), suggesting IL-6-mediated bone marrow release of neutrophils. Furthermore, the increases in urine MPO concentration were correlated with increases in urine IL-6 (r = 0.868, P < 0.01) and G-CSF (r = 0.875, P < 0.01), suggesting that these cytokines promoted neutrophil activation in vivo. However, in vitro preincubation of neutrophils and monocytes with postexercise plasma could not cause priming responses, possibly because of the exercise-induced enhancement of plasma antioxidant activity. Conclusion: Although many cytokines recruiting and priming neutrophils and monocytes were secreted and functional after exhaustive exercise, overwhelming antioxidant and antiinflammatory defenses were induced, preventing exercise-induced oxidative stress.

Original languageEnglish
Pages (from-to)348-355
Number of pages8
JournalMedicine and Science in Sports and Exercise
Volume35
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1
Externally publishedYes

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Neutrophils
Cytokines
Interleukin-6
Exercise
Neutrophil Activation
Peroxidase
Lactoferrin
Urine
Monocytes
Chemokine CCL2
Granulocyte-Macrophage Colony-Stimulating Factor
Antioxidants
Luminol
Granulocyte Colony-Stimulating Factor
Luminescence
Interleukin-8
Interleukin-1
Interleukin-10
Oxidative Stress
Anti-Inflammatory Agents

Keywords

  • Adhesion molecules
  • Chemokines
  • Free radicals
  • Inflammation
  • Leukocytes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

Impact of a competitive marathon race on systemic cytokine and neutrophil responses. / Suzuki, Katsuhiko; Nakaji, Shigeyuki; Yamada, Mutsuo; Liu, Qiang; Kurakake, Shigeyoshi; Okamura, Noriyoshi; Kumae, Takashi; Umeda, Takashi; Sugawara, Kazuo.

In: Medicine and Science in Sports and Exercise, Vol. 35, No. 2, 01.02.2003, p. 348-355.

Research output: Contribution to journalArticle

Suzuki, K, Nakaji, S, Yamada, M, Liu, Q, Kurakake, S, Okamura, N, Kumae, T, Umeda, T & Sugawara, K 2003, 'Impact of a competitive marathon race on systemic cytokine and neutrophil responses', Medicine and Science in Sports and Exercise, vol. 35, no. 2, pp. 348-355. https://doi.org/10.1249/01.MSS.0000048861.57899.04
Suzuki, Katsuhiko ; Nakaji, Shigeyuki ; Yamada, Mutsuo ; Liu, Qiang ; Kurakake, Shigeyoshi ; Okamura, Noriyoshi ; Kumae, Takashi ; Umeda, Takashi ; Sugawara, Kazuo. / Impact of a competitive marathon race on systemic cytokine and neutrophil responses. In: Medicine and Science in Sports and Exercise. 2003 ; Vol. 35, No. 2. pp. 348-355.
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AU - Okamura, Noriyoshi

AU - Kumae, Takashi

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