Improved passive cell distributing method for micro cellular diagnostic well array

Kentaro Kawai, Masaki Kanai, Hiroaki Nakanishi, Shuichi Shoji

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    We developed a novel biological cell injection method for a large number of diagnostic wells fabricated in parallel. This method facilitates simultaneous cell injection into a microwell array with a simple channel configuration, which can distribute cells evenly to each microwell only by introducing cell suspensions from the inlet. In order to enhance the uniformity of the numbers of injected cells in each microwell, we developed the new concept of flow interchange, which can suppress deviation of the cell flow profile in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated using prototype devices of eight microwell arrays. It was confirmed that the flow interchange structure is effective to realize uniform injection. The coefficient of variation of bead numbers in eight microwells was 20.7%.

    Original languageEnglish
    Pages (from-to)5607-5613
    Number of pages7
    JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
    Volume45
    Issue number6 B
    DOIs
    Publication statusPublished - 2006 Jun 20

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    Keywords

    • μTAS
    • Cell sorting
    • High throughput screening
    • Micro well array
    • Passive distribution

    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

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