Improved passive cell distributing method to micro cellular diagnostic well array

Kentaro Kawai; Masaki Kanai; Hiroaki Nakanishi; Shuichi Shoji

doi:10.1109/imnc.2005.203731

Improved passive cell distributing method to micro cellular diagnostic well array

Kentaro Kawai, Masaki Kanai, Hiroaki Nakanishi, Shuichi Shoji

School of Fundamental Science and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We developed a novel cell injection method to the microwell array, which can distribute cells evenly to each microwell only by introducing cell suspension from the inlet. This method facilitates simultaneous cell injection into a number of diagnostic well with simple channel configuration. In order to enhance the uniformity of injected cell numbers in each microwell, we propose a new concept of the flow interchange, which can suppress deviation of the cell concentration in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated in prototype devices of 8 microwell array. It was confirmed that the cross interchange structure is effective to improve the uniformity compared to the previous microchannel. The coefficient of variation between the 8 microwells was 20.7%.

Original language	English
Title of host publication	Digest of Papers - Microprocesses and Nanotechnology 2005
Subtitle of host publication	2005 International Microprocesses and Nanotechnology Conference
Publisher	IEEE Computer Society
Pages	48-49
Number of pages	2
ISBN (Print)	4990247221, 9784990247225
DOIs	https://doi.org/10.1109/imnc.2005.203731
Publication status	Published - 2005
Event	2005 International Microprocesses and Nanotechnology Conference - Tokyo, Japan Duration: 2005 Oct 25 → 2005 Oct 28

Publication series

Name	Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference
Volume	2005

Conference

Conference	2005 International Microprocesses and Nanotechnology Conference
Country	Japan
City	Tokyo
Period	05/10/25 → 05/10/28

Keywords

Cell sorting
High throughput screening
Micro well array
Passive distribution

ASJC Scopus subject areas

Engineering(all)

Access to Document

10.1109/imnc.2005.203731

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Cite this

Kawai, K., Kanai, M., Nakanishi, H., & Shoji, S. (2005). Improved passive cell distributing method to micro cellular diagnostic well array. In Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference (pp. 48-49). [1595207] (Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference; Vol. 2005). IEEE Computer Society. https://doi.org/10.1109/imnc.2005.203731

Improved passive cell distributing method to micro cellular diagnostic well array. / Kawai, Kentaro; Kanai, Masaki; Nakanishi, Hiroaki; Shoji, Shuichi.

Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. IEEE Computer Society, 2005. p. 48-49 1595207 (Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference; Vol. 2005).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kawai, K, Kanai, M, Nakanishi, H & Shoji, S 2005, Improved passive cell distributing method to micro cellular diagnostic well array. in Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference., 1595207, Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference, vol. 2005, IEEE Computer Society, pp. 48-49, 2005 International Microprocesses and Nanotechnology Conference, Tokyo, Japan, 05/10/25. https://doi.org/10.1109/imnc.2005.203731

Kawai K, Kanai M, Nakanishi H, Shoji S. Improved passive cell distributing method to micro cellular diagnostic well array. In Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. IEEE Computer Society. 2005. p. 48-49. 1595207. (Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference). https://doi.org/10.1109/imnc.2005.203731

Kawai, Kentaro ; Kanai, Masaki ; Nakanishi, Hiroaki ; Shoji, Shuichi. / Improved passive cell distributing method to micro cellular diagnostic well array. Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. IEEE Computer Society, 2005. pp. 48-49 (Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference).

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