Improved passive cell distributing method to micro cellular diagnostic well array

Kentaro Kawai, Masaki Kanai, Hiroaki Nakanishi, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We developed a novel cell injection method to the microwell array, which can distribute cells evenly to each microwell only by introducing cell suspension from the inlet. This method facilitates simultaneous cell injection into a number of diagnostic well with simple channel configuration. In order to enhance the uniformity of injected cell numbers in each microwell, we propose a new concept of the flow interchange, which can suppress deviation of the cell concentration in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated in prototype devices of 8 microwell array. It was confirmed that the cross interchange structure is effective to improve the uniformity compared to the previous microchannel. The coefficient of variation between the 8 microwells was 20.7%.

    Original languageEnglish
    Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference
    Pages48-49
    Number of pages2
    Volume2005
    Publication statusPublished - 2005
    Event2005 International Microprocesses and Nanotechnology Conference - Tokyo
    Duration: 2005 Oct 252005 Oct 28

    Other

    Other2005 International Microprocesses and Nanotechnology Conference
    CityTokyo
    Period05/10/2505/10/28

    Fingerprint

    Interchanges
    Microchannels

    Keywords

    • Cell sorting
    • High throughput screening
    • Micro well array
    • Passive distribution

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

    Kawai, K., Kanai, M., Nakanishi, H., & Shoji, S. (2005). Improved passive cell distributing method to micro cellular diagnostic well array. In Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference (Vol. 2005, pp. 48-49). [1595207]

    Improved passive cell distributing method to micro cellular diagnostic well array. / Kawai, Kentaro; Kanai, Masaki; Nakanishi, Hiroaki; Shoji, Shuichi.

    Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. Vol. 2005 2005. p. 48-49 1595207.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Kawai, K, Kanai, M, Nakanishi, H & Shoji, S 2005, Improved passive cell distributing method to micro cellular diagnostic well array. in Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. vol. 2005, 1595207, pp. 48-49, 2005 International Microprocesses and Nanotechnology Conference, Tokyo, 05/10/25.
    Kawai K, Kanai M, Nakanishi H, Shoji S. Improved passive cell distributing method to micro cellular diagnostic well array. In Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. Vol. 2005. 2005. p. 48-49. 1595207
    Kawai, Kentaro ; Kanai, Masaki ; Nakanishi, Hiroaki ; Shoji, Shuichi. / Improved passive cell distributing method to micro cellular diagnostic well array. Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. Vol. 2005 2005. pp. 48-49
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