Improved passive cell distributing method to micro cellular diagnostic well array

Kentaro Kawai, Masaki Kanai, Hiroaki Nakanishi, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a novel cell injection method to the microwell array, which can distribute cells evenly to each microwell only by introducing cell suspension from the inlet. This method facilitates simultaneous cell injection into a number of diagnostic well with simple channel configuration. In order to enhance the uniformity of injected cell numbers in each microwell, we propose a new concept of the flow interchange, which can suppress deviation of the cell concentration in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated in prototype devices of 8 microwell array. It was confirmed that the cross interchange structure is effective to improve the uniformity compared to the previous microchannel. The coefficient of variation between the 8 microwells was 20.7%.

Original languageEnglish
Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2005
Subtitle of host publication2005 International Microprocesses and Nanotechnology Conference
PublisherIEEE Computer Society
Pages48-49
Number of pages2
ISBN (Print)4990247221, 9784990247225
DOIs
Publication statusPublished - 2005
Event2005 International Microprocesses and Nanotechnology Conference - Tokyo, Japan
Duration: 2005 Oct 252005 Oct 28

Publication series

NameDigest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference
Volume2005

Conference

Conference2005 International Microprocesses and Nanotechnology Conference
CountryJapan
CityTokyo
Period05/10/2505/10/28

Keywords

  • Cell sorting
  • High throughput screening
  • Micro well array
  • Passive distribution

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Kawai, K., Kanai, M., Nakanishi, H., & Shoji, S. (2005). Improved passive cell distributing method to micro cellular diagnostic well array. In Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference (pp. 48-49). [1595207] (Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference; Vol. 2005). IEEE Computer Society. https://doi.org/10.1109/imnc.2005.203731