Abstract
We developed a novel cell injection method to the microwell array, which can distribute cells evenly to each microwell only by introducing cell suspension from the inlet. This method facilitates simultaneous cell injection into a number of diagnostic well with simple channel configuration. In order to enhance the uniformity of injected cell numbers in each microwell, we propose a new concept of the flow interchange, which can suppress deviation of the cell concentration in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated in prototype devices of 8 microwell array. It was confirmed that the cross interchange structure is effective to improve the uniformity compared to the previous microchannel. The coefficient of variation between the 8 microwells was 20.7%.
| Original language | English |
|---|---|
| Title of host publication | Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference |
| Pages | 48-49 |
| Number of pages | 2 |
| Volume | 2005 |
| Publication status | Published - 2005 |
| Event | 2005 International Microprocesses and Nanotechnology Conference - Tokyo Duration: 2005 Oct 25 → 2005 Oct 28 |
Other
| Other | 2005 International Microprocesses and Nanotechnology Conference |
|---|---|
| City | Tokyo |
| Period | 05/10/25 → 05/10/28 |
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Keywords
- Cell sorting
- High throughput screening
- Micro well array
- Passive distribution
ASJC Scopus subject areas
- Engineering(all)
Cite this
Improved passive cell distributing method to micro cellular diagnostic well array. / Kawai, Kentaro; Kanai, Masaki; Nakanishi, Hiroaki; Shoji, Shuichi.
Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference. Vol. 2005 2005. p. 48-49 1595207.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Improved passive cell distributing method to micro cellular diagnostic well array
AU - Kawai, Kentaro
AU - Kanai, Masaki
AU - Nakanishi, Hiroaki
AU - Shoji, Shuichi
PY - 2005
Y1 - 2005
N2 - We developed a novel cell injection method to the microwell array, which can distribute cells evenly to each microwell only by introducing cell suspension from the inlet. This method facilitates simultaneous cell injection into a number of diagnostic well with simple channel configuration. In order to enhance the uniformity of injected cell numbers in each microwell, we propose a new concept of the flow interchange, which can suppress deviation of the cell concentration in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated in prototype devices of 8 microwell array. It was confirmed that the cross interchange structure is effective to improve the uniformity compared to the previous microchannel. The coefficient of variation between the 8 microwells was 20.7%.
AB - We developed a novel cell injection method to the microwell array, which can distribute cells evenly to each microwell only by introducing cell suspension from the inlet. This method facilitates simultaneous cell injection into a number of diagnostic well with simple channel configuration. In order to enhance the uniformity of injected cell numbers in each microwell, we propose a new concept of the flow interchange, which can suppress deviation of the cell concentration in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated in prototype devices of 8 microwell array. It was confirmed that the cross interchange structure is effective to improve the uniformity compared to the previous microchannel. The coefficient of variation between the 8 microwells was 20.7%.
KW - Cell sorting
KW - High throughput screening
KW - Micro well array
KW - Passive distribution
UR - http://www.scopus.com/inward/record.url?scp=33847217671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847217671&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33847217671
SN - 4990247221
SN - 9784990247225
VL - 2005
SP - 48
EP - 49
BT - Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference
ER -