Improvement in citric acid production by haploidization of Aspergillus niger diploid strains

Kotaro Kirimura, Pyo Lee Sung Pyo Lee, Iwao Nakajima, Seiichiro Kawabe, Shoji Usami

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Segregation of diploid strains by a haploidizing agent was used to improve citric acid producing strains of Aspergillus niger. Stable diploid strains were obtained via protoplast fusion between two citric acid-producing strains from different genealogies, one for shaking culture and the other for solid culture. Diploid strains were treated by benomyl as a haploidizing agent, and many segregants were obtained. Prototrophic segregants were selected and their haploidy was confirmed by their conidial size and DNA contents. The prototrophic segregants were very variable in their citric acid productivities, some of them better either in shaking culture or in solid culture than both the parental strains. The presence of methanol stimulated citric acid production by the parental and the diploid strains. However, all prototrophic segregants derived from one diploid strain had higher productivities in solid cultures without methanol than in those with methanol.

    Original languageEnglish
    Pages (from-to)375-382
    Number of pages8
    JournalJournal of Fermentation Technology
    Volume66
    Issue number4
    DOIs
    Publication statusPublished - 1988

    Fingerprint

    Aspergillus niger
    Aspergillus
    Citric acid
    Diploidy
    Citric Acid
    Methanol
    Benomyl
    Genealogy and Heraldry
    Protoplasts
    Haploidy
    Productivity
    DNA
    Fusion reactions

    Cite this

    Improvement in citric acid production by haploidization of Aspergillus niger diploid strains. / Kirimura, Kotaro; Sung Pyo Lee, Pyo Lee; Nakajima, Iwao; Kawabe, Seiichiro; Usami, Shoji.

    In: Journal of Fermentation Technology, Vol. 66, No. 4, 1988, p. 375-382.

    Research output: Contribution to journalArticle

    Kirimura, Kotaro ; Sung Pyo Lee, Pyo Lee ; Nakajima, Iwao ; Kawabe, Seiichiro ; Usami, Shoji. / Improvement in citric acid production by haploidization of Aspergillus niger diploid strains. In: Journal of Fermentation Technology. 1988 ; Vol. 66, No. 4. pp. 375-382.
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    AU - Kawabe, Seiichiro

    AU - Usami, Shoji

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