Improvement of denitrifying reaction rate by enhanced substrate transport within a cell-immobilized space by electrophoresis

Satoshi Tsuneda, Shuichi Kaku, Hiroshi Hayashi, Satoshi Ohgushi, Akihiko Terada, Akira Hirata

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    In order to improve the microbial reaction rate within a cell-immobilized space, this study experimentally investigated enhanced substrate transport by electrophoresis of a specific ion. Batch-mode and continuous feeding denitrification tests were carried out in an autotrophic denitrifying bioreactor using sulfur-oxidizing microbes as a model system. Seed sludge and powdered sulfur were immobilized on fibrous carrier medium, and an electric field was applied through the cell-immobilized zone via inert electrodes on both sides of vessel. In batch-mode denitrification tests, nitrogen removal rate reached 1.99 g-N·m-3·h-1 when the electric field of 18V was reversed at intervals of 30min, as compared with 0.40g-N·m -3·h-1 when the electric field was not applied. These findings demonstrated that the denitrification rate was increased by promoting the transport of NO3 - ions across the cell-immobilized zone by electrophoresis. In addition, no reduction of NO 3 - to NO2 - occurred, indicating that decrease of nitrogen compound was due to biological reaction. Mathematical simulation analysis using a model involving Monod-type reaction kinetics and the Stokes formula for NO3 - mobility was in general agreement with the experimental results. The results of the continuous feeding test also indicated that electrophoresis improved the nitrogen removal rate.

    Original languageEnglish
    Pages (from-to)507-513
    Number of pages7
    JournalKagaku Kogaku Ronbunshu
    Volume32
    Issue number6
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Denitrification
    Electrophoresis
    Reaction rates
    Nitrogen removal
    Electric fields
    Sulfur
    Substrates
    Ions
    Nitrogen Compounds
    Nitrogen compounds
    Bioreactors
    Reaction kinetics
    Seed
    Electrodes

    Keywords

    • Bacterial cell immobilization
    • Biological wastewater treatment
    • Denitrification
    • Electrophoresis
    • Fibrous slag

    ASJC Scopus subject areas

    • Materials Science (miscellaneous)

    Cite this

    Improvement of denitrifying reaction rate by enhanced substrate transport within a cell-immobilized space by electrophoresis. / Tsuneda, Satoshi; Kaku, Shuichi; Hayashi, Hiroshi; Ohgushi, Satoshi; Terada, Akihiko; Hirata, Akira.

    In: Kagaku Kogaku Ronbunshu, Vol. 32, No. 6, 2006, p. 507-513.

    Research output: Contribution to journalArticle

    Tsuneda, Satoshi ; Kaku, Shuichi ; Hayashi, Hiroshi ; Ohgushi, Satoshi ; Terada, Akihiko ; Hirata, Akira. / Improvement of denitrifying reaction rate by enhanced substrate transport within a cell-immobilized space by electrophoresis. In: Kagaku Kogaku Ronbunshu. 2006 ; Vol. 32, No. 6. pp. 507-513.
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