Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system

Tomoyasu Atoji, Motonari Aihara, Shinji Takeoka, Eishun Tsuchida

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

    Original languageEnglish
    Title of host publicationPolymer Preprints, Japan
    Pages4927-4928
    Number of pages2
    Volume54
    Edition2
    Publication statusPublished - 2005
    Event54th SPSJ Symposium on Macromolecules - Yamagata
    Duration: 2005 Sep 202005 Sep 22

    Other

    Other54th SPSJ Symposium on Macromolecules
    CityYamagata
    Period05/9/2005/9/22

    Fingerprint

    Hemoglobin
    Oxygen
    Phospholipids
    Hydrogen peroxide
    Membranes
    Hydrogen
    Experiments

    Keywords

    • Hb vesicles
    • Hydrogen peroxide/
    • L-tyrosine
    • MetHb
    • Peroxidase activity

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

    Atoji, T., Aihara, M., Takeoka, S., & Tsuchida, E. (2005). Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system. In Polymer Preprints, Japan (2 ed., Vol. 54, pp. 4927-4928)

    Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system. / Atoji, Tomoyasu; Aihara, Motonari; Takeoka, Shinji; Tsuchida, Eishun.

    Polymer Preprints, Japan. Vol. 54 2. ed. 2005. p. 4927-4928.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Atoji, T, Aihara, M, Takeoka, S & Tsuchida, E 2005, Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system. in Polymer Preprints, Japan. 2 edn, vol. 54, pp. 4927-4928, 54th SPSJ Symposium on Macromolecules, Yamagata, 05/9/20.
    Atoji T, Aihara M, Takeoka S, Tsuchida E. Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system. In Polymer Preprints, Japan. 2 ed. Vol. 54. 2005. p. 4927-4928
    Atoji, Tomoyasu ; Aihara, Motonari ; Takeoka, Shinji ; Tsuchida, Eishun. / Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system. Polymer Preprints, Japan. Vol. 54 2. ed. 2005. pp. 4927-4928
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    AU - Takeoka, Shinji

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