Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system

Tomoyasu Atoji, Motonari Aihara, Shinji Takeoka, Eishun Tsuchida

Research output: Contribution to conferencePaper

Abstract

Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

Original languageEnglish
Pages4927-4928
Number of pages2
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

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Keywords

  • Hb vesicles
  • Hydrogen peroxide/
  • L-tyrosine
  • MetHb
  • Peroxidase activity

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Atoji, T., Aihara, M., Takeoka, S., & Tsuchida, E. (2005). Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system. 4927-4928. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.