Improvement of protein binding capacity of acrylic-acid-grafted fibers by polymer root-to-brush shift

Yuka Matsuzaki, Takeshi Itabashi, Shigeko Kawai-Noma, Daisuke Umeno, Kyoichi Saito

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A weak acid cation exchange fiber used for large-scale antibody purification was prepared by radiation-induced graft polymerization of acrylic acid (AA) onto a commercially available nylon-6 fiber. The AA-grafted fiber was post-treated by conditioning in NaOH aqueous solution at 298 K followed by immersion in water at 353 K to increase the protein binding capacity of the cation exchange fiber. A 2 g/L lysozyme solution buffered at pH 6.0 flowed through a column charged with a treated or untreated AA-grafted fiber at a space velocity of 20 h −1 . The 10% dynamic binding capacity of the column charged with the former fiber of 200 mg/mL-column was 5.9-fold that of the column charged with the latter fiber, and 2.3-fold that (88 mg/mL-column) of a column charged with CM Sepharose™ Fast Flow (GE Healthcare).

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalRadiation Physics and Chemistry
Volume158
DOIs
Publication statusPublished - 2019 May
Externally publishedYes

Keywords

  • Acrylic-acid-grafted fiber
  • Conditioning with alkaline solution
  • Immersion in hot water
  • Protein-binding capacity
  • Radiation-induced graft polymerization

ASJC Scopus subject areas

  • Radiation

Fingerprint Dive into the research topics of 'Improvement of protein binding capacity of acrylic-acid-grafted fibers by polymer root-to-brush shift'. Together they form a unique fingerprint.

Cite this