Abstract
We investigated the signal-to-noise ratio (S/N) of real-time single-molecule fluorescence imaging (SMFI) using zero-mode waveguides (ZMWs). The excitation light and the fluorescence propagating from a molecule in the ZMW were analyzed by computational optics simulation. The dependence of the S/N on the ZMW structure was investigated with the diameter and etching depth as the simulation parameters. We found that the SMFI using a conventional ZMW was near the critical level for detecting binding and dissociation events. We show that etching the glass surface of the ZMW by 60 nm enhances the S/N six times the conventional nonetched ZMWs. The enhanced S/N improves the temporal resolution of the SMFI at physiological concentrations.
| Original language | English |
|---|---|
| Article number | 012727 |
| Journal | Physical Review E - Statistical, Nonlinear, and Soft Matter Physics |
| Volume | 88 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 Jul 30 |
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ASJC Scopus subject areas
- Condensed Matter Physics
- Statistical and Nonlinear Physics
- Statistics and Probability
- Medicine(all)
Cite this
Improving zero-mode waveguide structure for enhancing signal-to-noise ratio of real-time single-molecule fluorescence imaging : A computational study. / Tanii, Takashi; Akahori, Rena; Higano, Shun; Okubo, Kotaro; Yamamoto, Hideaki; Ueno, Taro; Funatsu, Takashi.
In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 88, No. 1, 012727, 30.07.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improving zero-mode waveguide structure for enhancing signal-to-noise ratio of real-time single-molecule fluorescence imaging
T2 - A computational study
AU - Tanii, Takashi
AU - Akahori, Rena
AU - Higano, Shun
AU - Okubo, Kotaro
AU - Yamamoto, Hideaki
AU - Ueno, Taro
AU - Funatsu, Takashi
PY - 2013/7/30
Y1 - 2013/7/30
N2 - We investigated the signal-to-noise ratio (S/N) of real-time single-molecule fluorescence imaging (SMFI) using zero-mode waveguides (ZMWs). The excitation light and the fluorescence propagating from a molecule in the ZMW were analyzed by computational optics simulation. The dependence of the S/N on the ZMW structure was investigated with the diameter and etching depth as the simulation parameters. We found that the SMFI using a conventional ZMW was near the critical level for detecting binding and dissociation events. We show that etching the glass surface of the ZMW by 60 nm enhances the S/N six times the conventional nonetched ZMWs. The enhanced S/N improves the temporal resolution of the SMFI at physiological concentrations.
AB - We investigated the signal-to-noise ratio (S/N) of real-time single-molecule fluorescence imaging (SMFI) using zero-mode waveguides (ZMWs). The excitation light and the fluorescence propagating from a molecule in the ZMW were analyzed by computational optics simulation. The dependence of the S/N on the ZMW structure was investigated with the diameter and etching depth as the simulation parameters. We found that the SMFI using a conventional ZMW was near the critical level for detecting binding and dissociation events. We show that etching the glass surface of the ZMW by 60 nm enhances the S/N six times the conventional nonetched ZMWs. The enhanced S/N improves the temporal resolution of the SMFI at physiological concentrations.
UR - http://www.scopus.com/inward/record.url?scp=84881159692&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881159692&partnerID=8YFLogxK
U2 - 10.1103/PhysRevE.88.012727
DO - 10.1103/PhysRevE.88.012727
M3 - Article
VL - 88
JO - Physical review. E
JF - Physical review. E
SN - 1539-3755
IS - 1
M1 - 012727
ER -