Improving zero-mode waveguide structure for enhancing signal-to-noise ratio of real-time single-molecule fluorescence imaging

A computational study

Takashi Tanii, Rena Akahori, Shun Higano, Kotaro Okubo, Hideaki Yamamoto, Taro Ueno, Takashi Funatsu

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    We investigated the signal-to-noise ratio (S/N) of real-time single-molecule fluorescence imaging (SMFI) using zero-mode waveguides (ZMWs). The excitation light and the fluorescence propagating from a molecule in the ZMW were analyzed by computational optics simulation. The dependence of the S/N on the ZMW structure was investigated with the diameter and etching depth as the simulation parameters. We found that the SMFI using a conventional ZMW was near the critical level for detecting binding and dissociation events. We show that etching the glass surface of the ZMW by 60 nm enhances the S/N six times the conventional nonetched ZMWs. The enhanced S/N improves the temporal resolution of the SMFI at physiological concentrations.

    Original languageEnglish
    Article number012727
    JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
    Volume88
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jul 30

    Fingerprint

    Optical Imaging
    Signal-To-Noise Ratio
    Fluorescence
    Waveguide
    signal to noise ratios
    Imaging
    waveguides
    Real-time
    fluorescence
    Zero
    molecules
    Etching
    Glass
    etching
    Light
    temporal resolution
    Single Molecule Imaging
    Optics
    Simulation
    simulation

    ASJC Scopus subject areas

    • Condensed Matter Physics
    • Statistical and Nonlinear Physics
    • Statistics and Probability
    • Medicine(all)

    Cite this

    Improving zero-mode waveguide structure for enhancing signal-to-noise ratio of real-time single-molecule fluorescence imaging : A computational study. / Tanii, Takashi; Akahori, Rena; Higano, Shun; Okubo, Kotaro; Yamamoto, Hideaki; Ueno, Taro; Funatsu, Takashi.

    In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 88, No. 1, 012727, 30.07.2013.

    Research output: Contribution to journalArticle

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    AU - Okubo, Kotaro

    AU - Yamamoto, Hideaki

    AU - Ueno, Taro

    AU - Funatsu, Takashi

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