Abstract
The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes - Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling.
| Original language | English |
|---|---|
| Pages (from-to) | 2827-2839 |
| Number of pages | 13 |
| Journal | Marine Drugs |
| Volume | 12 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Actinomycetes
- Antibiotics
- In situ detection
- Raman microspectroscopy
- Secondary metabolites
ASJC Scopus subject areas
- Drug Discovery
- Medicine(all)
Cite this
In situ detection of antibiotic amphotericin B produced in Streptomyces nodosus using Raman microspectroscopy. / Miyaoka, Rimi; Hosokawa, Masahito; Ando, Masahiro; Mori, Tetsushi; Hamaguchi, Hiro O.; Takeyama, Haruko.
In: Marine Drugs, Vol. 12, No. 5, 2014, p. 2827-2839.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In situ detection of antibiotic amphotericin B produced in Streptomyces nodosus using Raman microspectroscopy
AU - Miyaoka, Rimi
AU - Hosokawa, Masahito
AU - Ando, Masahiro
AU - Mori, Tetsushi
AU - Hamaguchi, Hiro O.
AU - Takeyama, Haruko
PY - 2014
Y1 - 2014
N2 - The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes - Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling.
AB - The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes - Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling.
KW - Actinomycetes
KW - Antibiotics
KW - In situ detection
KW - Raman microspectroscopy
KW - Secondary metabolites
UR - http://www.scopus.com/inward/record.url?scp=84901472515&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901472515&partnerID=8YFLogxK
U2 - 10.3390/md12052827
DO - 10.3390/md12052827
M3 - Article
C2 - 24828290
AN - SCOPUS:84901472515
VL - 12
SP - 2827
EP - 2839
JO - Marine Drugs
JF - Marine Drugs
SN - 1660-3397
IS - 5
ER -