In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers

Hideaki Yamamoto, Kazunori Okano, Takanori Demura, Yoichiroh Hosokawa, Hiroshi Masuhara, Takashi Tanii, Shun Nakamura

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19 Citations (Scopus)


In-situ guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks in vitro.

Original languageEnglish
Article number163701
JournalApplied Physics Letters
Issue number16
Publication statusPublished - 2011 Oct 17


ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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