In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers

Hideaki Yamamoto, Kazunori Okano, Takanori Demura, Yoichiroh Hosokawa, Hiroshi Masuhara, Takashi Tanii, Shun Nakamura

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    In-situ guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks in vitro.

    Original languageEnglish
    Article number163701
    JournalApplied Physics Letters
    Volume99
    Issue number16
    DOIs
    Publication statusPublished - 2011 Oct 17

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    adhesion
    scanning
    cells
    neurons
    laser ablation
    lasers
    templates
    laser beams
    adsorption
    molecules

    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

    Cite this

    In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers. / Yamamoto, Hideaki; Okano, Kazunori; Demura, Takanori; Hosokawa, Yoichiroh; Masuhara, Hiroshi; Tanii, Takashi; Nakamura, Shun.

    In: Applied Physics Letters, Vol. 99, No. 16, 163701, 17.10.2011.

    Research output: Contribution to journalArticle

    Yamamoto, Hideaki ; Okano, Kazunori ; Demura, Takanori ; Hosokawa, Yoichiroh ; Masuhara, Hiroshi ; Tanii, Takashi ; Nakamura, Shun. / In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers. In: Applied Physics Letters. 2011 ; Vol. 99, No. 16.
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