In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall

Hisataka Maruyama, Yu Matsuda, Tomohide Niimi, Nobuyuki Unozumi, Kei Nanatani, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier layer. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier layer to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation of photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1570-1572
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Fluorescence
  • Micro chamber
  • Photosynthesis
  • Single cell measurement

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Maruyama, H., Matsuda, Y., Niimi, T., Unozumi, N., Nanatani, K., & Arai, F. (2012). In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1570-1572). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.