In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall

Hisataka Maruyama, yu Matsuda, Tomohide Niimi, Nobuyuki Unozumi, Kei Nanatani, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier layer. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier layer to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation of photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1570-1572
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Photosynthesis
Gases
Oxygen sensors
Oxygen
Photodegradation
Dissolved oxygen
Sugars
Fluorescence

Keywords

  • Fluorescence
  • Micro chamber
  • Photosynthesis
  • Single cell measurement

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Maruyama, H., Matsuda, Y., Niimi, T., Unozumi, N., Nanatani, K., & Arai, F. (2012). In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1570-1572). Chemical and Biological Microsystems Society.

In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall. / Maruyama, Hisataka; Matsuda, yu; Niimi, Tomohide; Unozumi, Nobuyuki; Nanatani, Kei; Arai, F.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1570-1572.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Maruyama, H, Matsuda, Y, Niimi, T, Unozumi, N, Nanatani, K & Arai, F 2012, In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1570-1572, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, 12/10/28.
Maruyama H, Matsuda Y, Niimi T, Unozumi N, Nanatani K, Arai F. In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1570-1572
Maruyama, Hisataka ; Matsuda, yu ; Niimi, Tomohide ; Unozumi, Nobuyuki ; Nanatani, Kei ; Arai, F. / In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1570-1572
@inproceedings{e0011679ce084604afc9b4d34c6e7be1,
title = "In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall",
abstract = "We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier layer. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier layer to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation of photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.",
keywords = "Fluorescence, Micro chamber, Photosynthesis, Single cell measurement",
author = "Hisataka Maruyama and yu Matsuda and Tomohide Niimi and Nobuyuki Unozumi and Kei Nanatani and F. Arai",
year = "2012",
month = "1",
day = "1",
language = "English",
isbn = "9780979806452",
pages = "1570--1572",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - In-situ measurement of photosynthesis using single synecocystis sp. PCC 6803 in a microchamber with gas barrier wall

AU - Maruyama, Hisataka

AU - Matsuda, yu

AU - Niimi, Tomohide

AU - Unozumi, Nobuyuki

AU - Nanatani, Kei

AU - Arai, F.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier layer. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier layer to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation of photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.

AB - We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier layer. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier layer to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation of photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.

KW - Fluorescence

KW - Micro chamber

KW - Photosynthesis

KW - Single cell measurement

UR - http://www.scopus.com/inward/record.url?scp=84901787225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901787225&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9780979806452

SP - 1570

EP - 1572

BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - Chemical and Biological Microsystems Society

ER -