In situ modification of cell-culture scaffolds by photocatalysis of visible-light-responsive TiO2 film

Sho Kono, Kohei Furusawa, Atsushi Kurotobi, Kohei Hattori, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii

Research output: Contribution to journalArticlepeer-review

Abstract

We propose a novel process to modify the cell affinity of scaffolds in a cell-culture environment using the photocatalytic activity of visible-light (VL)-responsive TiO2. The proposed process is the improved version of our previous demonstration in which ultraviolet (UV)-responsive TiO2 was utilized. In that demonstration, we showed that cell-repellent molecules on TiO2 were decomposed and replaced with cell-permissive molecules upon UV exposure in the medium where cells are being cultured. However, UV irradiation involves taking the risk of inducing damage to the cells. In this work, a TiO2 film was sputter-deposited on a quartz coverslip at 640 °C without O2 gas injection to create a rutile structure containing oxygen defects, which is known to exhibit photocatalytic activity upon VL exposure. We show that the cell adhesion site and migration area can be controlled with the photocatalytic activity of the VL-responsive TiO2 film, while the cellular oxidative stress is reduced markedly by the substitution of VL for UV.

Original languageEnglish
Article number027001
JournalJapanese journal of applied physics
Volume57
Issue number2
DOIs
Publication statusPublished - 2018 Feb

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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