In situ modification of cell-culture scaffolds by photocatalytic decomposition of organosilane monolayers

Hideaki Yamamoto, Takanori Demura, Mayu Morita, Sho Kono, Kohei Sekine, Takahiro Shinada, Shun Nakamura, Takashi Tanii

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    We demonstrate a novel application of TiO2 photocatalysis for modifying the cell affinity of a scaffold surface in a cell-culture environment. An as-deposited octadecyltrichlorosilane self-assembled monolayer (OTS SAM) on TiO2 was found to be hydrophobic and stably adsorbed serum albumins that blocked subsequent adsorption of other proteins and cells. Upon irradiation of ultraviolet (UV) light, OTS molecules were decomposed and became permissive to the adhesion of PC12 cells via adsorption of an extracellular matrix protein, collagen. Optimal UV dose was 200 J cm-2 for OTS SAM on TiO2. The amount of collagen adsorption decreased when excessive UV light was irradiated, most likely due to the surface being too hydrophilic to support its adsorption. This UV-induced modification required TiO2 to be present under the SAM and hence is a result of TiO2 photocatalysis. The UV irradiation for surface modification can be performed before cell plating or during cell culture. We also demonstrate that poly(ethylene glycol) SAM can also be patterned with this method, indicating that it is applicable to both hydrophobic and hydrophilic SAMs. This method provides a unique tool for fabricating cell microarrays and studying dynamical properties of living cells.

    Original languageEnglish
    Article number35021
    JournalBiofabrication
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Scaffolds (biology)
    Cell culture
    Monolayers
    Cell Culture Techniques
    Adsorption
    Decomposition
    Photocatalysis
    Self assembled monolayers
    Collagen
    Ultraviolet Rays
    Irradiation
    Proteins
    Extracellular Matrix Proteins
    Microarrays
    Plating
    Serum Albumin
    Ethylene Glycol
    Polyethylene glycols
    PC12 Cells
    Surface treatment

    Keywords

    • Cell patterning
    • Photocatalysis
    • Protein patterning
    • Self-assembled monolayer
    • Titanium dioxide

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biochemistry
    • Biomaterials
    • Biomedical Engineering

    Cite this

    In situ modification of cell-culture scaffolds by photocatalytic decomposition of organosilane monolayers. / Yamamoto, Hideaki; Demura, Takanori; Morita, Mayu; Kono, Sho; Sekine, Kohei; Shinada, Takahiro; Nakamura, Shun; Tanii, Takashi.

    In: Biofabrication, Vol. 6, No. 3, 35021, 2014.

    Research output: Contribution to journalArticle

    Yamamoto, Hideaki ; Demura, Takanori ; Morita, Mayu ; Kono, Sho ; Sekine, Kohei ; Shinada, Takahiro ; Nakamura, Shun ; Tanii, Takashi. / In situ modification of cell-culture scaffolds by photocatalytic decomposition of organosilane monolayers. In: Biofabrication. 2014 ; Vol. 6, No. 3.
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