In situ telomerase activity in pleural effusions: A promising marker for malignancy

Annika Dejmek, Naoyuki Yahata, Kazuma Ohyashiki, Yoshiro Ebihara, Masatoshi Kakihana, Takashi Hirano, Norihiko Kawate, Harubumi Kato

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25 Citations (Scopus)

Abstract

Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.

Original languageEnglish
Pages (from-to)11-15
Number of pages5
JournalDiagnostic Cytopathology
Volume24
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Keywords

  • Cytology
  • Effusions
  • Telomerase
  • TRAP in situ

ASJC Scopus subject areas

  • Anatomy

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