In situ transplantation of adipose tissue-derived stem cells organized on porous polymer nanosheets for murine skin defects

Keisuke Nishiwaki, Shimpo Aoki, Manabu Kinoshita, Tomoharu Kiyosawa, Yoshitaka Suematsu, Shinji Takeoka, Toshinori Fujie

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Stem cell transplantation is expected to be an effective early-phase treatment for deep burn injuries and intractable ulcers. Localizing and proliferating stem cells on the lesion utilizing engineered scaffolds is important for this treatment. In this study, we demonstrated in situ transplantation of adipose-tissue derived stem cells (ASCs) organized on free-standing porous polymer ultrathin films (referred to as “porous nanosheets”) to a skin defect model in diabetic mice. Porous nanosheets were prepared by a combination of micro-gravure coating with macrophase separation of poly(d,l-lactic acid) and polystyrene under a roll-to-roll process and solvent etching process with cyclohexane. The permeable structure of porous nanosheets (thickness of 150 nm, average pore diameter of 4 μm) allowed for proliferation of ASCs and also provided sufficient nutrient inflow into multilayered ASC constructs. Then, transplantation of a trilayered ASC-laden porous nanosheet achieved homogeneous transference of ASCs onto the skin lesion. Transplanted ASCs contributed to wound healing in a dorsal skin defect model in diabetic mice. Thus, cell transplantation using porous nanosheets will be a new method for promoting wound healing in diabetic and other kinds of refractory ulcers.

Original languageEnglish
Pages (from-to)1363-1371
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume107
Issue number5
DOIs
Publication statusPublished - 2019 Jul

Keywords

  • adipose-tissue derived stem cells (ASCs)
  • cell transplantation
  • diabetic db/db mouse
  • polymer nanosheet
  • porous structure

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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