TY - JOUR
T1 - In situ transplantation of adipose tissue-derived stem cells organized on porous polymer nanosheets for murine skin defects
AU - Nishiwaki, Keisuke
AU - Aoki, Shimpo
AU - Kinoshita, Manabu
AU - Kiyosawa, Tomoharu
AU - Suematsu, Yoshitaka
AU - Takeoka, Shinji
AU - Fujie, Toshinori
N1 - Funding Information:
This work was supported in part by the Precursory Research for Embryonic Science and Technology (PRESTO) from the Japan Science and Technology Agency (No. JPMJPR152A); JSPS KAKENHI (No. 15H05355, 17 K20116 and 18H05469 for T.F., No. 15 K10961 for S.A.); Waseda University Grant for Special Research Projects (Project number: 2017 K-393); the Noguchi Institute; the Tanaka Kikinzoku Memorial Foundation.
Funding Information:
Additional Supporting Information may be found in the online version of this article. Correspondence to: T. Fujie; e-mail: t.fujie@aoni.waseda.jp Contract grant sponsor: Tanaka Kikinzoku Memorial Foundation Contract grant sponsor: Noguchi Institute Contract grant sponsor: Waseda University Grant for Special Research Projects; contract grant number: 2017K-393 Contract grant sponsor: JSPS KAKENHI; contract grant number: 15K10961, 18H05469, 17K20116, 15H05355 Contract grant sponsor: Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency; contract grant number: JPMJPR152A
Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2019/7
Y1 - 2019/7
N2 - Stem cell transplantation is expected to be an effective early-phase treatment for deep burn injuries and intractable ulcers. Localizing and proliferating stem cells on the lesion utilizing engineered scaffolds is important for this treatment. In this study, we demonstrated in situ transplantation of adipose-tissue derived stem cells (ASCs) organized on free-standing porous polymer ultrathin films (referred to as “porous nanosheets”) to a skin defect model in diabetic mice. Porous nanosheets were prepared by a combination of micro-gravure coating with macrophase separation of poly(d,l-lactic acid) and polystyrene under a roll-to-roll process and solvent etching process with cyclohexane. The permeable structure of porous nanosheets (thickness of 150 nm, average pore diameter of 4 μm) allowed for proliferation of ASCs and also provided sufficient nutrient inflow into multilayered ASC constructs. Then, transplantation of a trilayered ASC-laden porous nanosheet achieved homogeneous transference of ASCs onto the skin lesion. Transplanted ASCs contributed to wound healing in a dorsal skin defect model in diabetic mice. Thus, cell transplantation using porous nanosheets will be a new method for promoting wound healing in diabetic and other kinds of refractory ulcers.
AB - Stem cell transplantation is expected to be an effective early-phase treatment for deep burn injuries and intractable ulcers. Localizing and proliferating stem cells on the lesion utilizing engineered scaffolds is important for this treatment. In this study, we demonstrated in situ transplantation of adipose-tissue derived stem cells (ASCs) organized on free-standing porous polymer ultrathin films (referred to as “porous nanosheets”) to a skin defect model in diabetic mice. Porous nanosheets were prepared by a combination of micro-gravure coating with macrophase separation of poly(d,l-lactic acid) and polystyrene under a roll-to-roll process and solvent etching process with cyclohexane. The permeable structure of porous nanosheets (thickness of 150 nm, average pore diameter of 4 μm) allowed for proliferation of ASCs and also provided sufficient nutrient inflow into multilayered ASC constructs. Then, transplantation of a trilayered ASC-laden porous nanosheet achieved homogeneous transference of ASCs onto the skin lesion. Transplanted ASCs contributed to wound healing in a dorsal skin defect model in diabetic mice. Thus, cell transplantation using porous nanosheets will be a new method for promoting wound healing in diabetic and other kinds of refractory ulcers.
KW - adipose-tissue derived stem cells (ASCs)
KW - cell transplantation
KW - diabetic db/db mouse
KW - polymer nanosheet
KW - porous structure
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U2 - 10.1002/jbm.b.34228
DO - 10.1002/jbm.b.34228
M3 - Article
C2 - 30265776
AN - SCOPUS:85054097970
SN - 1552-4973
VL - 107
SP - 1363
EP - 1371
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
IS - 5
ER -