In utero and lactational dioxin exposure induces Sema3b and Sema3g gene expression in the developing mouse brain

Eiki Kimura, Toshihiro Endo, Wataru Yoshioka, Yunjie Ding, Waka Ujita, Masaki Kakeyama, Chiharu Tohyama

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    In the developing mammalian brain, neural network formation is regulated by complex signaling cascades. In utero and lactational dioxin exposure is known to induce higher brain function abnormalities and dendritic growth disruption in rodents. However, it is unclear whether perinatal dioxin exposure affects the expression of genes involved in neural network formation. Therefore, we investigated changes in gene expression in the brain regions of developing mice born to dams administered 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dose: 0, 0.6, or 3.0 μg/kg) on gestational day 12.5. Quantitative RT-PCR showed that TCDD exposure induced Ahrr expression in the cerebral cortex, hippocampus, and olfactory bulb of 3-day-old mice. Gene microarray analysis indicated that the mRNA expression levels of Sema3b and Sema3g, which encode proteins that are known to control axonal projections, were elevated in the olfactory bulb of TCDD-exposed mice, and the induction of these genes was observed during a 2-week postnatal period. Increased Sema3g expression was also observed in the brain but not in the kidney, liver, lung, and spleen of TCDD-exposed neonatal mice. These results indicate that the Sema3b and Sema3g genes are sensitive to brain-specific induction by dioxin exposure, which may disrupt neural network formation in the mammalian nervous system, thereby leading to abnormal higher brain function in adulthood.

    Original languageEnglish
    Pages (from-to)108-113
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume476
    Issue number2
    DOIs
    Publication statusPublished - 2016 Jul 22

    Fingerprint

    Dioxins
    Gene expression
    Brain
    Gene Expression
    Genes
    Olfactory Bulb
    Neural networks
    Neurology
    Microarray Analysis
    Microarrays
    Liver
    Cerebral Cortex
    Nervous System
    Dams
    Rodentia
    Hippocampus
    Spleen
    Polychlorinated Dibenzodioxins
    Kidney
    Polymerase Chain Reaction

    Keywords

    • Brain development
    • Developmental neurotoxicity
    • Dioxin
    • Olfactory bulb
    • Semaphorin

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Molecular Biology

    Cite this

    In utero and lactational dioxin exposure induces Sema3b and Sema3g gene expression in the developing mouse brain. / Kimura, Eiki; Endo, Toshihiro; Yoshioka, Wataru; Ding, Yunjie; Ujita, Waka; Kakeyama, Masaki; Tohyama, Chiharu.

    In: Biochemical and Biophysical Research Communications, Vol. 476, No. 2, 22.07.2016, p. 108-113.

    Research output: Contribution to journalArticle

    Kimura, Eiki ; Endo, Toshihiro ; Yoshioka, Wataru ; Ding, Yunjie ; Ujita, Waka ; Kakeyama, Masaki ; Tohyama, Chiharu. / In utero and lactational dioxin exposure induces Sema3b and Sema3g gene expression in the developing mouse brain. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 476, No. 2. pp. 108-113.
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