In utero and lactational exposure to low doses of chlorinated and brominated dioxins induces deficits in the fear memory of male mice

Asahi Haijima, Toshihiro Endo, Yan Zhang, Wataru Miyazaki, Masaki Kakeyama, Chiharu Tohyama

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Environmental-level in utero and lactational exposures to dioxins have been considered to affect brain functions of offspring. Here, we determined whether in utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 2,3,7,8-tetrabromodibenzo-p-dioxin (TBDD), at the dose that does not harm the dams, affects the acquisition and retention of fear memory in mouse offspring. Pregnant C57BL/6J mice were administered by gavages TCDD or TBDD at a dose of 0 or 3.0μg/kg body weight on gestation day 12.5, and their male offspring were examined for their behavior in adulthood. In the fear conditioning, a paired presentation of tone and foot shock was repeated three times, and retention tests for contextual and auditory fear memory were carried out 1 and 24. h after the fear conditioning. Groups of mice that were exposed to TCDD and TBDD in utero and via lactation showed deficits in the contextual and auditory retention tests at 1 and 24. h retention intervals. The present results suggest that maternal exposure to a low dose of TCDD or TBDD disrupts the functions of memory and emotion in male mouse offspring, and that the developmental toxicities of these chemicals are similar to each other.

Original languageEnglish
Pages (from-to)385-390
Number of pages6
JournalNeuroToxicology
Volume31
Issue number4
DOIs
Publication statusPublished - 2010 Aug
Externally publishedYes

Fingerprint

Dioxins
Fear
Data storage equipment
Maternal Exposure
Inbred C57BL Mouse
Lactation
Foot
Shock
Emotions
Body Weight
Pregnancy
Dams
Toxicity
Retention (Psychology)
Polychlorinated Dibenzodioxins
1,4-dioxin
Brain
Conditioning (Psychology)

Keywords

  • 2,3,7,8-tetrabromodibenzo-p-dioxin
  • 2,3,7,8-tetrachlorodibenzo-p-dioxin
  • Fear conditioning
  • In utero and lactational exposure
  • Mice

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

In utero and lactational exposure to low doses of chlorinated and brominated dioxins induces deficits in the fear memory of male mice. / Haijima, Asahi; Endo, Toshihiro; Zhang, Yan; Miyazaki, Wataru; Kakeyama, Masaki; Tohyama, Chiharu.

In: NeuroToxicology, Vol. 31, No. 4, 08.2010, p. 385-390.

Research output: Contribution to journalArticle

Haijima, Asahi ; Endo, Toshihiro ; Zhang, Yan ; Miyazaki, Wataru ; Kakeyama, Masaki ; Tohyama, Chiharu. / In utero and lactational exposure to low doses of chlorinated and brominated dioxins induces deficits in the fear memory of male mice. In: NeuroToxicology. 2010 ; Vol. 31, No. 4. pp. 385-390.
@article{b6764424ae9d4d87bd50eb43a3020895,
title = "In utero and lactational exposure to low doses of chlorinated and brominated dioxins induces deficits in the fear memory of male mice",
abstract = "Environmental-level in utero and lactational exposures to dioxins have been considered to affect brain functions of offspring. Here, we determined whether in utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 2,3,7,8-tetrabromodibenzo-p-dioxin (TBDD), at the dose that does not harm the dams, affects the acquisition and retention of fear memory in mouse offspring. Pregnant C57BL/6J mice were administered by gavages TCDD or TBDD at a dose of 0 or 3.0μg/kg body weight on gestation day 12.5, and their male offspring were examined for their behavior in adulthood. In the fear conditioning, a paired presentation of tone and foot shock was repeated three times, and retention tests for contextual and auditory fear memory were carried out 1 and 24. h after the fear conditioning. Groups of mice that were exposed to TCDD and TBDD in utero and via lactation showed deficits in the contextual and auditory retention tests at 1 and 24. h retention intervals. The present results suggest that maternal exposure to a low dose of TCDD or TBDD disrupts the functions of memory and emotion in male mouse offspring, and that the developmental toxicities of these chemicals are similar to each other.",
keywords = "2,3,7,8-tetrabromodibenzo-p-dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin, Fear conditioning, In utero and lactational exposure, Mice",
author = "Asahi Haijima and Toshihiro Endo and Yan Zhang and Wataru Miyazaki and Masaki Kakeyama and Chiharu Tohyama",
year = "2010",
month = "8",
doi = "10.1016/j.neuro.2010.04.004",
language = "English",
volume = "31",
pages = "385--390",
journal = "NeuroToxicology",
issn = "0161-813X",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - In utero and lactational exposure to low doses of chlorinated and brominated dioxins induces deficits in the fear memory of male mice

AU - Haijima, Asahi

AU - Endo, Toshihiro

AU - Zhang, Yan

AU - Miyazaki, Wataru

AU - Kakeyama, Masaki

AU - Tohyama, Chiharu

PY - 2010/8

Y1 - 2010/8

N2 - Environmental-level in utero and lactational exposures to dioxins have been considered to affect brain functions of offspring. Here, we determined whether in utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 2,3,7,8-tetrabromodibenzo-p-dioxin (TBDD), at the dose that does not harm the dams, affects the acquisition and retention of fear memory in mouse offspring. Pregnant C57BL/6J mice were administered by gavages TCDD or TBDD at a dose of 0 or 3.0μg/kg body weight on gestation day 12.5, and their male offspring were examined for their behavior in adulthood. In the fear conditioning, a paired presentation of tone and foot shock was repeated three times, and retention tests for contextual and auditory fear memory were carried out 1 and 24. h after the fear conditioning. Groups of mice that were exposed to TCDD and TBDD in utero and via lactation showed deficits in the contextual and auditory retention tests at 1 and 24. h retention intervals. The present results suggest that maternal exposure to a low dose of TCDD or TBDD disrupts the functions of memory and emotion in male mouse offspring, and that the developmental toxicities of these chemicals are similar to each other.

AB - Environmental-level in utero and lactational exposures to dioxins have been considered to affect brain functions of offspring. Here, we determined whether in utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 2,3,7,8-tetrabromodibenzo-p-dioxin (TBDD), at the dose that does not harm the dams, affects the acquisition and retention of fear memory in mouse offspring. Pregnant C57BL/6J mice were administered by gavages TCDD or TBDD at a dose of 0 or 3.0μg/kg body weight on gestation day 12.5, and their male offspring were examined for their behavior in adulthood. In the fear conditioning, a paired presentation of tone and foot shock was repeated three times, and retention tests for contextual and auditory fear memory were carried out 1 and 24. h after the fear conditioning. Groups of mice that were exposed to TCDD and TBDD in utero and via lactation showed deficits in the contextual and auditory retention tests at 1 and 24. h retention intervals. The present results suggest that maternal exposure to a low dose of TCDD or TBDD disrupts the functions of memory and emotion in male mouse offspring, and that the developmental toxicities of these chemicals are similar to each other.

KW - 2,3,7,8-tetrabromodibenzo-p-dioxin

KW - 2,3,7,8-tetrachlorodibenzo-p-dioxin

KW - Fear conditioning

KW - In utero and lactational exposure

KW - Mice

UR - http://www.scopus.com/inward/record.url?scp=77953545216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953545216&partnerID=8YFLogxK

U2 - 10.1016/j.neuro.2010.04.004

DO - 10.1016/j.neuro.2010.04.004

M3 - Article

VL - 31

SP - 385

EP - 390

JO - NeuroToxicology

JF - NeuroToxicology

SN - 0161-813X

IS - 4

ER -