In vitro cleavage of Drosophila 2S rRNA by M1 RNA.

Y. Hori, T. Tanaka, Yo Kikuchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Drosophila melanogaster initiator methionine tRNA can adopt an alternative conformation in aqueous solution. In this alternative conformation, the aminoacyl- and the anticodon stems of tRNA are unfolded and then these unfolded regions are used to form extended D- and T-stems, resulting in the formation of two tandemly joined stems and loops. This conformational alternation was recognized then cleaved by the catalytic RNA of Escherichia coli ribonuclease P (M1 RNA). The cleavage occurs within the mature sequence of tRNA. This further processing within mature sequence is called hyperprocessing. During the screening experiments of other conformational changeable D. melanogaster tRNAs by M1 RNA, we incidentally found that M1 RNA also hyperprocessed D. melanogaster 2S rRNA. Kinetic analyses of the hyperprocessing reaction of 2S rRNA by M1 RNA revealed that 2S rRNA could form a homodimer.

Original languageEnglish
Pages (from-to)93-94
Number of pages2
JournalNucleic acids symposium series
Issue number44
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Drosophila
Transfer RNA
Drosophila melanogaster
RNA
Ribonuclease P
RNA, Transfer, Met
Anticodon
Catalytic RNA
Methionine
Escherichia coli
In Vitro Techniques

Cite this

Hori, Y., Tanaka, T., & Kikuchi, Y. (2000). In vitro cleavage of Drosophila 2S rRNA by M1 RNA. Nucleic acids symposium series, (44), 93-94.

In vitro cleavage of Drosophila 2S rRNA by M1 RNA. / Hori, Y.; Tanaka, T.; Kikuchi, Yo.

In: Nucleic acids symposium series, No. 44, 2000, p. 93-94.

Research output: Contribution to journalArticle

Hori, Y, Tanaka, T & Kikuchi, Y 2000, 'In vitro cleavage of Drosophila 2S rRNA by M1 RNA.', Nucleic acids symposium series, no. 44, pp. 93-94.
Hori, Y. ; Tanaka, T. ; Kikuchi, Yo. / In vitro cleavage of Drosophila 2S rRNA by M1 RNA. In: Nucleic acids symposium series. 2000 ; No. 44. pp. 93-94.
@article{6e3c2a8f23ae42be88feda3138e896af,
title = "In vitro cleavage of Drosophila 2S rRNA by M1 RNA.",
abstract = "Drosophila melanogaster initiator methionine tRNA can adopt an alternative conformation in aqueous solution. In this alternative conformation, the aminoacyl- and the anticodon stems of tRNA are unfolded and then these unfolded regions are used to form extended D- and T-stems, resulting in the formation of two tandemly joined stems and loops. This conformational alternation was recognized then cleaved by the catalytic RNA of Escherichia coli ribonuclease P (M1 RNA). The cleavage occurs within the mature sequence of tRNA. This further processing within mature sequence is called hyperprocessing. During the screening experiments of other conformational changeable D. melanogaster tRNAs by M1 RNA, we incidentally found that M1 RNA also hyperprocessed D. melanogaster 2S rRNA. Kinetic analyses of the hyperprocessing reaction of 2S rRNA by M1 RNA revealed that 2S rRNA could form a homodimer.",
author = "Y. Hori and T. Tanaka and Yo Kikuchi",
year = "2000",
language = "English",
pages = "93--94",
journal = "Nucleic acids symposium series",
issn = "0261-3166",
publisher = "Information Retrieval",
number = "44",

}

TY - JOUR

T1 - In vitro cleavage of Drosophila 2S rRNA by M1 RNA.

AU - Hori, Y.

AU - Tanaka, T.

AU - Kikuchi, Yo

PY - 2000

Y1 - 2000

N2 - Drosophila melanogaster initiator methionine tRNA can adopt an alternative conformation in aqueous solution. In this alternative conformation, the aminoacyl- and the anticodon stems of tRNA are unfolded and then these unfolded regions are used to form extended D- and T-stems, resulting in the formation of two tandemly joined stems and loops. This conformational alternation was recognized then cleaved by the catalytic RNA of Escherichia coli ribonuclease P (M1 RNA). The cleavage occurs within the mature sequence of tRNA. This further processing within mature sequence is called hyperprocessing. During the screening experiments of other conformational changeable D. melanogaster tRNAs by M1 RNA, we incidentally found that M1 RNA also hyperprocessed D. melanogaster 2S rRNA. Kinetic analyses of the hyperprocessing reaction of 2S rRNA by M1 RNA revealed that 2S rRNA could form a homodimer.

AB - Drosophila melanogaster initiator methionine tRNA can adopt an alternative conformation in aqueous solution. In this alternative conformation, the aminoacyl- and the anticodon stems of tRNA are unfolded and then these unfolded regions are used to form extended D- and T-stems, resulting in the formation of two tandemly joined stems and loops. This conformational alternation was recognized then cleaved by the catalytic RNA of Escherichia coli ribonuclease P (M1 RNA). The cleavage occurs within the mature sequence of tRNA. This further processing within mature sequence is called hyperprocessing. During the screening experiments of other conformational changeable D. melanogaster tRNAs by M1 RNA, we incidentally found that M1 RNA also hyperprocessed D. melanogaster 2S rRNA. Kinetic analyses of the hyperprocessing reaction of 2S rRNA by M1 RNA revealed that 2S rRNA could form a homodimer.

UR - http://www.scopus.com/inward/record.url?scp=0141670280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141670280&partnerID=8YFLogxK

M3 - Article

C2 - 12903284

AN - SCOPUS:0141670280

SP - 93

EP - 94

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

IS - 44

ER -