In-vitro effects of mammalian and amphibian prolactins on hepatic vitellogenin synthesis in Rana esculenta

O. Carnevali, G. Mosconi, K. Yamamoto, T. Kobayashi, S. Kikuyama, A. M. Polzonetti-Magni

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Abstract

Male and female Rana esculenta liver was induced in an in-vitro system by homologous and Rana catesbeiana pituitary to synthesize and release vitellogenin, a lipoglycophosphoprotein precursor of yolk proteins, lipovitellins and phosvitinis, in oviparous vertebrates. In the present experiments, the action of prolactin on hepatic vitellogenin synthesis and release was investigated, using ovine prolactin and Rana catesbeiana prolactin. The effects of prolactin on hepatic vitellogeninn synthesis displayed different trends related to sex; male liver was found to be more responsive than female liver to both ovine and frog prolactin; moreover, the response to prolactin was dose-related (r = 0.998; P < 0.05) in male but not in female liver. In both sexes, a high degree of seasonality in the responsiveness of the liver was found, since the vitellogenin levels induced by prolactin during the winter phase were significantly (P < 0.001) higher than those produced during the summer phase. Thus, there was no significant difference between the action of ovine and frog prolactin on vitellogenin synthesis; in fact, mammalian prolactins are structurally similar with regard to nucleotide and amino acid sequences. The direct action of prolactin on hepatic vitellogenin synthesis in the frog Rana esculenta is discussed, on the basis of the role played by prolactin as an important growth modulatory hormone in fetal and adult tissues.

Original languageEnglish
Pages (from-to)383-389
Number of pages7
JournalJournal of Endocrinology
Volume137
Issue number3
Publication statusPublished - 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Endocrinology

Cite this

Carnevali, O., Mosconi, G., Yamamoto, K., Kobayashi, T., Kikuyama, S., & Polzonetti-Magni, A. M. (1993). In-vitro effects of mammalian and amphibian prolactins on hepatic vitellogenin synthesis in Rana esculenta. Journal of Endocrinology, 137(3), 383-389.