In vitro electrochemical detection of wheat allergen using rat basophilic leukaemia (RBL-1) cells

N. Nakamura, S. Kumazawa, Tadashi Matsunaga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have investigated an electrochemical method of detecting foods that cause an allergic reaction. Rat basophilic leukaemia (RBL-1) cells were sensitized with serum from a rat that was allergic to wheat. A sample containing the protein fraction of a food was added to the cells and incubated. The cells were immobilized on a membrane filter and attached to a basalplane pyrolytic graphite electrode. When a potential was applied in the range 0–1.0 V relative to a saturated calomel electrode, an anodic peak current appeared at around 0.33 V. This peak current, attributed to serotonin, increased with time, and the maximum current (0.5 μA) was obtained 20–25 min of incubation. The response of the RBL-1 cells was specific to the protein fraction of wheat. The peak current increased linearly with increasing protein concentration in the range of 0.01–0.5 μg ml−1. These results suggest that the concentration of the protein bringing about the allergic reaction can be determined by cyclic voltammetry within 25 min. This method is more sensitive than the conventional skin tests.

Original languageEnglish
Pages (from-to)622-625
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume43
Issue number4
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Allergens
Triticum
Leukemia
Hypersensitivity
Electrodes
Proteins
Food
Immobilized Cells
Graphite
Skin Tests
Serotonin
Membranes
In Vitro Techniques
Serum

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

In vitro electrochemical detection of wheat allergen using rat basophilic leukaemia (RBL-1) cells. / Nakamura, N.; Kumazawa, S.; Matsunaga, Tadashi.

In: Applied Microbiology and Biotechnology, Vol. 43, No. 4, 1995, p. 622-625.

Research output: Contribution to journalArticle

Nakamura, N. ; Kumazawa, S. ; Matsunaga, Tadashi. / In vitro electrochemical detection of wheat allergen using rat basophilic leukaemia (RBL-1) cells. In: Applied Microbiology and Biotechnology. 1995 ; Vol. 43, No. 4. pp. 622-625.
@article{3d64726a8c674a09a2278276407154f3,
title = "In vitro electrochemical detection of wheat allergen using rat basophilic leukaemia (RBL-1) cells",
abstract = "We have investigated an electrochemical method of detecting foods that cause an allergic reaction. Rat basophilic leukaemia (RBL-1) cells were sensitized with serum from a rat that was allergic to wheat. A sample containing the protein fraction of a food was added to the cells and incubated. The cells were immobilized on a membrane filter and attached to a basalplane pyrolytic graphite electrode. When a potential was applied in the range 0–1.0 V relative to a saturated calomel electrode, an anodic peak current appeared at around 0.33 V. This peak current, attributed to serotonin, increased with time, and the maximum current (0.5 μA) was obtained 20–25 min of incubation. The response of the RBL-1 cells was specific to the protein fraction of wheat. The peak current increased linearly with increasing protein concentration in the range of 0.01–0.5 μg ml−1. These results suggest that the concentration of the protein bringing about the allergic reaction can be determined by cyclic voltammetry within 25 min. This method is more sensitive than the conventional skin tests.",
author = "N. Nakamura and S. Kumazawa and Tadashi Matsunaga",
year = "1995",
doi = "10.1007/BF00164764",
language = "English",
volume = "43",
pages = "622--625",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - In vitro electrochemical detection of wheat allergen using rat basophilic leukaemia (RBL-1) cells

AU - Nakamura, N.

AU - Kumazawa, S.

AU - Matsunaga, Tadashi

PY - 1995

Y1 - 1995

N2 - We have investigated an electrochemical method of detecting foods that cause an allergic reaction. Rat basophilic leukaemia (RBL-1) cells were sensitized with serum from a rat that was allergic to wheat. A sample containing the protein fraction of a food was added to the cells and incubated. The cells were immobilized on a membrane filter and attached to a basalplane pyrolytic graphite electrode. When a potential was applied in the range 0–1.0 V relative to a saturated calomel electrode, an anodic peak current appeared at around 0.33 V. This peak current, attributed to serotonin, increased with time, and the maximum current (0.5 μA) was obtained 20–25 min of incubation. The response of the RBL-1 cells was specific to the protein fraction of wheat. The peak current increased linearly with increasing protein concentration in the range of 0.01–0.5 μg ml−1. These results suggest that the concentration of the protein bringing about the allergic reaction can be determined by cyclic voltammetry within 25 min. This method is more sensitive than the conventional skin tests.

AB - We have investigated an electrochemical method of detecting foods that cause an allergic reaction. Rat basophilic leukaemia (RBL-1) cells were sensitized with serum from a rat that was allergic to wheat. A sample containing the protein fraction of a food was added to the cells and incubated. The cells were immobilized on a membrane filter and attached to a basalplane pyrolytic graphite electrode. When a potential was applied in the range 0–1.0 V relative to a saturated calomel electrode, an anodic peak current appeared at around 0.33 V. This peak current, attributed to serotonin, increased with time, and the maximum current (0.5 μA) was obtained 20–25 min of incubation. The response of the RBL-1 cells was specific to the protein fraction of wheat. The peak current increased linearly with increasing protein concentration in the range of 0.01–0.5 μg ml−1. These results suggest that the concentration of the protein bringing about the allergic reaction can be determined by cyclic voltammetry within 25 min. This method is more sensitive than the conventional skin tests.

UR - http://www.scopus.com/inward/record.url?scp=0029166943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029166943&partnerID=8YFLogxK

U2 - 10.1007/BF00164764

DO - 10.1007/BF00164764

M3 - Article

C2 - 7546602

AN - SCOPUS:0029166943

VL - 43

SP - 622

EP - 625

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 4

ER -