In vitro engineering of vascularized tissue surrogates

Katsuhisa Sakaguchi, Tatsuya Shimizu, Shigeto Horaguchi, Hidekazu Sekine, Masayuki Yamato, Mitsuo Umezu, Teruo Okano

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

In vitro scaling up of bioengineered tissues is known to be limited by diffusion issues, specifically a lack of vasculature. Here, we report a new strategy for preserving cell viability in three-dimensional tissues using cell sheet technology and a perfusion bioreactor having collagen-based microchannels. When triple-layer cardiac cell sheets are incubated within this bioreactor, endothelial cells in the cell sheets migrate to vascularize in the collagen gel, and finally connect with the microchannels. Medium readily flows into the cell sheets through the microchannels and the newly developed capillaries, while the cardiac construct shows simultaneous beating. When additional triple-layer cell sheets are repeatedly layered, new multi-layer construct spontaneously integrates and the resulting construct becomes a vascularized thick tissue. These results confirmed our method to fabricate in vitro vascularized tissue surrogates that overcomes engineered-tissue thickness limitations. The surrogates promise new therapies for damaged organs as well as new in vitro tissue models.

Original languageEnglish
Article number1316
JournalScientific reports
Volume3
DOIs
Publication statusPublished - 2013

ASJC Scopus subject areas

  • General

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    Sakaguchi, K., Shimizu, T., Horaguchi, S., Sekine, H., Yamato, M., Umezu, M., & Okano, T. (2013). In vitro engineering of vascularized tissue surrogates. Scientific reports, 3, [1316]. https://doi.org/10.1038/srep01316