In vitro hyperprocessing of tRNAs by Bacillus subtilis ribonuclease P RNA.

Y. Hori, T. Tanaka, Yo Kikuchi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We have previously reported that the catalytic RNA subunit of ribonuclease P (RNase P) of Escherichia coli (M1 RNA) cleaves Drosophila initiator methionine tRNA (tRNA(i)Met), alanine tRNA (tRNA(Ala)) and histidine tRNA (tRNA(His)) within the mature tRNA sequences to produce specific fragments. We call this further cleavage hyperprocessing. These cleavages were dependent on the occurrence of altered conformations of the tRNAs. Here, we found that the RNase P RNA of Bacillus subtilis can hyperprocess these three tRNAs at the same sites as does M1 RNA. The hyperprocessing activity may probably be common feature for Bacterial RNase P RNAs.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages209-210
Number of pages2
Edition1
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Ribonuclease P
Transfer RNA
Bacillus subtilis
RNA
RNA, Transfer, Met
RNA, Transfer, His
RNA, Transfer, Ala
Histidine
Alanine
Methionine
Drosophila
In Vitro Techniques
Escherichia coli

Cite this

Hori, Y., Tanaka, T., & Kikuchi, Y. (2001). In vitro hyperprocessing of tRNAs by Bacillus subtilis ribonuclease P RNA. In Nucleic acids research. Supplement (2001) (1 ed., pp. 209-210)

In vitro hyperprocessing of tRNAs by Bacillus subtilis ribonuclease P RNA. / Hori, Y.; Tanaka, T.; Kikuchi, Yo.

Nucleic acids research. Supplement (2001). 1. ed. 2001. p. 209-210.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hori, Y, Tanaka, T & Kikuchi, Y 2001, In vitro hyperprocessing of tRNAs by Bacillus subtilis ribonuclease P RNA. in Nucleic acids research. Supplement (2001). 1 edn, pp. 209-210.
Hori Y, Tanaka T, Kikuchi Y. In vitro hyperprocessing of tRNAs by Bacillus subtilis ribonuclease P RNA. In Nucleic acids research. Supplement (2001). 1 ed. 2001. p. 209-210
Hori, Y. ; Tanaka, T. ; Kikuchi, Yo. / In vitro hyperprocessing of tRNAs by Bacillus subtilis ribonuclease P RNA. Nucleic acids research. Supplement (2001). 1. ed. 2001. pp. 209-210
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