Abstract
Here we present an in vitro selection method to obtain peptide agonists for G protein-coupled receptors (GPCRs) using a water-in-oil (W/O) microdroplet platform. Using this method, we successfully identified novel peptide agonists for human somatostatin receptor subtype-2 (SSTR2) from a library of partially randomized SST sequences. This result demonstrates that our method will be a powerful platform for identifying novel peptide agonists for human GPCRs with known and unknown endogenous ligands.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 593-594 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country/Territory | Ireland |
| City | Dublin |
| Period | 16/10/9 → 16/10/13 |
Keywords
- G protein-coupled receptor
- Microdroplet
- Peptide agonist
- Yeast
ASJC Scopus subject areas
- Control and Systems Engineering