In vitro selection of novel peptide agonists for human somatostatin receptor subtype-2 using a water-in-oil microdroplet platform

T. Sakurai, R. Iizuka, Y. Nakamura, J. Ishii, A. Kondo, A. Iguchi, Donghyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we present an in vitro selection method to obtain peptide agonists for G protein-coupled receptors (GPCRs) using a water-in-oil (W/O) microdroplet platform. Using this method, we successfully identified novel peptide agonists for human somatostatin receptor subtype-2 (SSTR2) from a library of partially randomized SST sequences. This result demonstrates that our method will be a powerful platform for identifying novel peptide agonists for human GPCRs with known and unknown endogenous ligands.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages593-594
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Fingerprint

Peptides
Proteins
Water
Ligands
Oils

Keywords

  • G protein-coupled receptor
  • Microdroplet
  • Peptide agonist
  • Yeast

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Sakurai, T., Iizuka, R., Nakamura, Y., Ishii, J., Kondo, A., Iguchi, A., ... Funatsu, T. (2016). In vitro selection of novel peptide agonists for human somatostatin receptor subtype-2 using a water-in-oil microdroplet platform. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 593-594). Chemical and Biological Microsystems Society.

In vitro selection of novel peptide agonists for human somatostatin receptor subtype-2 using a water-in-oil microdroplet platform. / Sakurai, T.; Iizuka, R.; Nakamura, Y.; Ishii, J.; Kondo, A.; Iguchi, A.; Yoon, Donghyun; Sekiguchi, Tetsushi; Shoji, Shuichi; Funatsu, T.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 593-594.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sakurai, T, Iizuka, R, Nakamura, Y, Ishii, J, Kondo, A, Iguchi, A, Yoon, D, Sekiguchi, T, Shoji, S & Funatsu, T 2016, In vitro selection of novel peptide agonists for human somatostatin receptor subtype-2 using a water-in-oil microdroplet platform. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 593-594, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.
Sakurai T, Iizuka R, Nakamura Y, Ishii J, Kondo A, Iguchi A et al. In vitro selection of novel peptide agonists for human somatostatin receptor subtype-2 using a water-in-oil microdroplet platform. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 593-594
Sakurai, T. ; Iizuka, R. ; Nakamura, Y. ; Ishii, J. ; Kondo, A. ; Iguchi, A. ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Shoji, Shuichi ; Funatsu, T. / In vitro selection of novel peptide agonists for human somatostatin receptor subtype-2 using a water-in-oil microdroplet platform. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 593-594
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