In vitro wound-healing analytical system composed of a micro automated scratcher and oxygen gradient chamber

Hiroki Ota, Nobuyuki Tanaka, Kazuhiro Fukumori, Nobuhito Goda, Masayuki Yamato, Teruo Okano

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    This study described an in-vitro wound-healing analytical system composed of a micro automated scratcher and an oxygen gradient micro-chamber. Cell migration assay using sharps has been used for investigating wound healing process, tumor metastasis, and angiogenesis formation. However, the technique is unable to control the physical size of scratching, because scratching is created manually by handy sharps, such as a pipette yet. For obtaining scratches having a contact physical dimension, this study attempted to develop a highly accurate cell-scratching instrument and demonstrated more efficient and precise physiologically investigation on cell migration in a gas gradient micro-chamber to be performed. A micro automated scratcher, composed of X, Y, Z, and θ-axes linear actuators, a pressure sensor, and a micro comb fabricated by a three-dimensional (3D) printer, was developed. Actuators controlled X, Y, Z, and θ directions, and a pressure sensor detected the contact of comb and the bottom of cell culture chamber. This scratcher was able to create of scratches with a 150 μm-scale width at a standard deviation of less than 13%. This high controllability and reproducibility were able to provide a well-controlled cell scratching in an oxygen gas gradient chamber, which allowed cell migration to be analyzed under various oxygen tensions (0-150mmHg) working as a function of wound healing process in one test. As a result, a hypoxia condition from 4 to 10 mmHg was found to promote the closing of cell scratching caused by cell migrations. The proposed scratching system, which had a precise cell-scratching capability and an oxygen gradient micro device, would offer an efficient experimental platform for the future cell migration studies, which would contribute to drug screening, tumor translational research, and regenerative medicine.

    Original languageEnglish
    Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    PublisherChemical and Biological Microsystems Society
    Pages34-36
    Number of pages3
    ISBN (Print)9780979806452
    Publication statusPublished - 2012
    Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa
    Duration: 2012 Oct 282012 Nov 1

    Other

    Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    CityOkinawa
    Period12/10/2812/11/1

    Fingerprint

    Oxygen
    Pressure sensors
    Tumors
    3D printers
    Gases
    Linear actuators
    Controllability
    Cell culture
    Assays
    Screening
    Actuators
    Pharmaceutical Preparations
    Direction compound
    Regenerative Medicine

    Keywords

    • Diam
    • Feugiat
    • Nulla
    • Pulvinar

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Ota, H., Tanaka, N., Fukumori, K., Goda, N., Yamato, M., & Okano, T. (2012). In vitro wound-healing analytical system composed of a micro automated scratcher and oxygen gradient chamber. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 34-36). Chemical and Biological Microsystems Society.

    In vitro wound-healing analytical system composed of a micro automated scratcher and oxygen gradient chamber. / Ota, Hiroki; Tanaka, Nobuyuki; Fukumori, Kazuhiro; Goda, Nobuhito; Yamato, Masayuki; Okano, Teruo.

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 34-36.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Ota, H, Tanaka, N, Fukumori, K, Goda, N, Yamato, M & Okano, T 2012, In vitro wound-healing analytical system composed of a micro automated scratcher and oxygen gradient chamber. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 34-36, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, 12/10/28.
    Ota H, Tanaka N, Fukumori K, Goda N, Yamato M, Okano T. In vitro wound-healing analytical system composed of a micro automated scratcher and oxygen gradient chamber. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 34-36
    Ota, Hiroki ; Tanaka, Nobuyuki ; Fukumori, Kazuhiro ; Goda, Nobuhito ; Yamato, Masayuki ; Okano, Teruo. / In vitro wound-healing analytical system composed of a micro automated scratcher and oxygen gradient chamber. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 34-36
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    abstract = "This study described an in-vitro wound-healing analytical system composed of a micro automated scratcher and an oxygen gradient micro-chamber. Cell migration assay using sharps has been used for investigating wound healing process, tumor metastasis, and angiogenesis formation. However, the technique is unable to control the physical size of scratching, because scratching is created manually by handy sharps, such as a pipette yet. For obtaining scratches having a contact physical dimension, this study attempted to develop a highly accurate cell-scratching instrument and demonstrated more efficient and precise physiologically investigation on cell migration in a gas gradient micro-chamber to be performed. A micro automated scratcher, composed of X, Y, Z, and θ-axes linear actuators, a pressure sensor, and a micro comb fabricated by a three-dimensional (3D) printer, was developed. Actuators controlled X, Y, Z, and θ directions, and a pressure sensor detected the contact of comb and the bottom of cell culture chamber. This scratcher was able to create of scratches with a 150 μm-scale width at a standard deviation of less than 13{\%}. This high controllability and reproducibility were able to provide a well-controlled cell scratching in an oxygen gas gradient chamber, which allowed cell migration to be analyzed under various oxygen tensions (0-150mmHg) working as a function of wound healing process in one test. As a result, a hypoxia condition from 4 to 10 mmHg was found to promote the closing of cell scratching caused by cell migrations. The proposed scratching system, which had a precise cell-scratching capability and an oxygen gradient micro device, would offer an efficient experimental platform for the future cell migration studies, which would contribute to drug screening, tumor translational research, and regenerative medicine.",
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    AU - Ota, Hiroki

    AU - Tanaka, Nobuyuki

    AU - Fukumori, Kazuhiro

    AU - Goda, Nobuhito

    AU - Yamato, Masayuki

    AU - Okano, Teruo

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    N2 - This study described an in-vitro wound-healing analytical system composed of a micro automated scratcher and an oxygen gradient micro-chamber. Cell migration assay using sharps has been used for investigating wound healing process, tumor metastasis, and angiogenesis formation. However, the technique is unable to control the physical size of scratching, because scratching is created manually by handy sharps, such as a pipette yet. For obtaining scratches having a contact physical dimension, this study attempted to develop a highly accurate cell-scratching instrument and demonstrated more efficient and precise physiologically investigation on cell migration in a gas gradient micro-chamber to be performed. A micro automated scratcher, composed of X, Y, Z, and θ-axes linear actuators, a pressure sensor, and a micro comb fabricated by a three-dimensional (3D) printer, was developed. Actuators controlled X, Y, Z, and θ directions, and a pressure sensor detected the contact of comb and the bottom of cell culture chamber. This scratcher was able to create of scratches with a 150 μm-scale width at a standard deviation of less than 13%. This high controllability and reproducibility were able to provide a well-controlled cell scratching in an oxygen gas gradient chamber, which allowed cell migration to be analyzed under various oxygen tensions (0-150mmHg) working as a function of wound healing process in one test. As a result, a hypoxia condition from 4 to 10 mmHg was found to promote the closing of cell scratching caused by cell migrations. The proposed scratching system, which had a precise cell-scratching capability and an oxygen gradient micro device, would offer an efficient experimental platform for the future cell migration studies, which would contribute to drug screening, tumor translational research, and regenerative medicine.

    AB - This study described an in-vitro wound-healing analytical system composed of a micro automated scratcher and an oxygen gradient micro-chamber. Cell migration assay using sharps has been used for investigating wound healing process, tumor metastasis, and angiogenesis formation. However, the technique is unable to control the physical size of scratching, because scratching is created manually by handy sharps, such as a pipette yet. For obtaining scratches having a contact physical dimension, this study attempted to develop a highly accurate cell-scratching instrument and demonstrated more efficient and precise physiologically investigation on cell migration in a gas gradient micro-chamber to be performed. A micro automated scratcher, composed of X, Y, Z, and θ-axes linear actuators, a pressure sensor, and a micro comb fabricated by a three-dimensional (3D) printer, was developed. Actuators controlled X, Y, Z, and θ directions, and a pressure sensor detected the contact of comb and the bottom of cell culture chamber. This scratcher was able to create of scratches with a 150 μm-scale width at a standard deviation of less than 13%. This high controllability and reproducibility were able to provide a well-controlled cell scratching in an oxygen gas gradient chamber, which allowed cell migration to be analyzed under various oxygen tensions (0-150mmHg) working as a function of wound healing process in one test. As a result, a hypoxia condition from 4 to 10 mmHg was found to promote the closing of cell scratching caused by cell migrations. The proposed scratching system, which had a precise cell-scratching capability and an oxygen gradient micro device, would offer an efficient experimental platform for the future cell migration studies, which would contribute to drug screening, tumor translational research, and regenerative medicine.

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