Abstract
The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.
| Original language | English |
|---|---|
| Pages (from-to) | 354-356 |
| Number of pages | 3 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 108 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 Oct |
| Externally published | Yes |
Fingerprint
Keywords
- Aptamer
- Circularization
- Constitutive promoter
- In vivo expression
- Permuted intron-exon
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
In vivo circular RNA production using a constitutive promoter for high-level expression. / Umekage, So; Kikuchi, Yo.
In: Journal of Bioscience and Bioengineering, Vol. 108, No. 4, 10.2009, p. 354-356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo circular RNA production using a constitutive promoter for high-level expression
AU - Umekage, So
AU - Kikuchi, Yo
PY - 2009/10
Y1 - 2009/10
N2 - The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.
AB - The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.
KW - Aptamer
KW - Circularization
KW - Constitutive promoter
KW - In vivo expression
KW - Permuted intron-exon
UR - http://www.scopus.com/inward/record.url?scp=69049118044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69049118044&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2009.04.011
DO - 10.1016/j.jbiosc.2009.04.011
M3 - Article
C2 - 19716528
AN - SCOPUS:69049118044
VL - 108
SP - 354
EP - 356
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 4
ER -