In vivo circular RNA production using a constitutive promoter for high-level expression

So Umekage, Yo Kikuchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.

Original languageEnglish
Pages (from-to)354-356
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume108
Issue number4
DOIs
Publication statusPublished - 2009 Oct
Externally publishedYes

Keywords

  • Aptamer
  • Circularization
  • Constitutive promoter
  • In vivo expression
  • Permuted intron-exon

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

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