The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.
- Constitutive promoter
- In vivo expression
- Permuted intron-exon
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology