In vivo circular RNA production using a constitutive promoter for high-level expression

So Umekage; Yo Kikuchi

doi:10.1016/j.jbiosc.2009.04.011

In vivo circular RNA production using a constitutive promoter for high-level expression

So Umekage, Yo Kikuchi

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.

Original language	English
Pages (from-to)	354-356
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	108
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiosc.2009.04.011
Publication status	Published - 2009 Oct
Externally published	Yes

Keywords

Aptamer
Circularization
Constitutive promoter
In vivo expression
Permuted intron-exon

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology
Bioengineering

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abstract = "The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.",

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KW - Permuted intron-exon

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