In vivo circular RNA production using a constitutive promoter for high-level expression

So Umekage; Yo Kikuchi

doi:10.1016/j.jbiosc.2009.04.011

In vivo circular RNA production using a constitutive promoter for high-level expression

So Umekage^*, Yo Kikuchi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.

Original language	English
Pages (from-to)	354-356
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	108
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiosc.2009.04.011
Publication status	Published - 2009 Oct
Externally published	Yes

Keywords

Aptamer
Circularization
Constitutive promoter
In vivo expression
Permuted intron-exon

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology
Bioengineering

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10.1016/j.jbiosc.2009.04.011

Cite this

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title = "In vivo circular RNA production using a constitutive promoter for high-level expression",

abstract = "The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.",

keywords = "Aptamer, Circularization, Constitutive promoter, In vivo expression, Permuted intron-exon",

author = "So Umekage and Yo Kikuchi",

year = "2009",

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language = "English",

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T1 - In vivo circular RNA production using a constitutive promoter for high-level expression

AU - Umekage, So

AU - Kikuchi, Yo

PY - 2009/10

Y1 - 2009/10

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AB - The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter.

KW - Aptamer

KW - Circularization

KW - Constitutive promoter

KW - In vivo expression

KW - Permuted intron-exon

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M3 - Article

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AN - SCOPUS:69049118044

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JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

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ER -

In vivo circular RNA production using a constitutive promoter for high-level expression

Abstract

Keywords

ASJC Scopus subject areas

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