In Vivo Gold Complex Catalysis within Live Mice

Kazuki Tsubokura; Kenward K.H. Vong; Ambara R. Pradipta; Akihiro Ogura; Sayaka Urano; Tsuyoshi Tahara; Satoshi Nozaki; Hirotaka Onoe; Yoichi Nakao; Regina Sibgatullina; Almira Kurbangalieva; Yasuyoshi Watanabe; Katsunori Tanaka

doi:10.1002/anie.201610273

In Vivo Gold Complex Catalysis within Live Mice

Kazuki Tsubokura, Kenward K.H. Vong, Ambara R. Pradipta, Akihiro Ogura, Sayaka Urano, Tsuyoshi Tahara, Satoshi Nozaki, Hirotaka Onoe, Yoichi Nakao, Regina Sibgatullina, Almira Kurbangalieva, Yasuyoshi Watanabe, Katsunori Tanaka^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

99 Citations (Scopus)

Abstract

Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin–Au^III complex was designed and developed that enables organ-specific, localized propargyl ester amidation with nearby proteins within live mice. The targeted reactivity can be imaged through the use of Cy7.5- and TAMRA-linked propargyl ester based fluorescent probes. This targeting system could enable the exploitation of other metal catalysis strategies for biomedical and clinical applications.

Original language	English
Pages (from-to)	3579-3584
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	56
Issue number	13
DOIs	https://doi.org/10.1002/anie.201610273
Publication status	Published - 2017 Mar 20

Keywords

amide bond formation
fluorescent labeling
glycoalbumin
gold catalysis

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201610273

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title = "In Vivo Gold Complex Catalysis within Live Mice",

abstract = "Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin–AuIII complex was designed and developed that enables organ-specific, localized propargyl ester amidation with nearby proteins within live mice. The targeted reactivity can be imaged through the use of Cy7.5- and TAMRA-linked propargyl ester based fluorescent probes. This targeting system could enable the exploitation of other metal catalysis strategies for biomedical and clinical applications.",

keywords = "amide bond formation, fluorescent labeling, glycoalbumin, gold catalysis",

author = "Kazuki Tsubokura and Vong, {Kenward K.H.} and Pradipta, {Ambara R.} and Akihiro Ogura and Sayaka Urano and Tsuyoshi Tahara and Satoshi Nozaki and Hirotaka Onoe and Yoichi Nakao and Regina Sibgatullina and Almira Kurbangalieva and Yasuyoshi Watanabe and Katsunori Tanaka",

note = "Funding Information: This work was supported by JSPS KAKENHI Grants (JP16H03287, JP16K13104, and JP15H05843) in Middle Molecular Strategy and by a subsidy from the Russian Government “Program of Competitive Growth of Kazan Federal University among World's Leading Academic Centers”. We thank Glytech, Inc. for supplying N-glycans. Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

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doi = "10.1002/anie.201610273",

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AU - Tsubokura, Kazuki

AU - Vong, Kenward K.H.

AU - Pradipta, Ambara R.

AU - Ogura, Akihiro

AU - Urano, Sayaka

AU - Tahara, Tsuyoshi

AU - Nozaki, Satoshi

AU - Onoe, Hirotaka

AU - Nakao, Yoichi

AU - Sibgatullina, Regina

AU - Kurbangalieva, Almira

AU - Watanabe, Yasuyoshi

AU - Tanaka, Katsunori

N1 - Funding Information: This work was supported by JSPS KAKENHI Grants (JP16H03287, JP16K13104, and JP15H05843) in Middle Molecular Strategy and by a subsidy from the Russian Government “Program of Competitive Growth of Kazan Federal University among World's Leading Academic Centers”. We thank Glytech, Inc. for supplying N-glycans. Publisher Copyright: © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2017/3/20

Y1 - 2017/3/20

N2 - Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin–AuIII complex was designed and developed that enables organ-specific, localized propargyl ester amidation with nearby proteins within live mice. The targeted reactivity can be imaged through the use of Cy7.5- and TAMRA-linked propargyl ester based fluorescent probes. This targeting system could enable the exploitation of other metal catalysis strategies for biomedical and clinical applications.

AB - Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin–AuIII complex was designed and developed that enables organ-specific, localized propargyl ester amidation with nearby proteins within live mice. The targeted reactivity can be imaged through the use of Cy7.5- and TAMRA-linked propargyl ester based fluorescent probes. This targeting system could enable the exploitation of other metal catalysis strategies for biomedical and clinical applications.

KW - amide bond formation

KW - fluorescent labeling

KW - glycoalbumin

KW - gold catalysis

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In Vivo Gold Complex Catalysis within Live Mice

Abstract

Keywords

ASJC Scopus subject areas

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