In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy

Masahito Hosokawa, Masahiro Ando, Shoichiro Mukai, Kyoko Osada, Tomoko Yoshino, Hiro O. Hamaguchi, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A straightforward in vivo monitoring technique for biomolecules would be an advantageous approach for understanding their spatiotemporal dynamics in living cells. However, the lack of adequate probes has hampered the quantitative determination of the chemical composition and metabolomics of cellular lipids at single-cell resolution. Here, we describe a method for the rapid, direct, and quantitative determination of lipid molecules from living cells using single-cell Raman imaging. In vivo localization of lipids in the form of triacylglycerol (TAG) within oleaginous microalga and their molecular compositions are monitored with high spatial resolution in a nondestructive and label-free manner. This method can provide quantitative and real-time information on compositions, chain lengths, and degree of unsaturation of fatty acids in living cells for improving the cultivating parameters or for determining the harvest timing during large-scale cultivations for microalgal lipid accumulation toward biodiesel production. Therefore, this technique is a potential tool for in vivo lipidomics for understanding the dynamics of lipid metabolisms in various organisms.

Original languageEnglish
Pages (from-to)8224-8230
Number of pages7
JournalAnalytical Chemistry
Volume86
Issue number16
DOIs
Publication statusPublished - 2014 Aug 19

Fingerprint

Lipids
Imaging techniques
Monitoring
Cells
Chemical analysis
Biofuels
Biomolecules
Chain length
Labels
Triglycerides
Fatty Acids
Molecules
Metabolomics
Lipid Metabolism

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Hosokawa, M., Ando, M., Mukai, S., Osada, K., Yoshino, T., Hamaguchi, H. O., & Tanaka, T. (2014). In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy. Analytical Chemistry, 86(16), 8224-8230. https://doi.org/10.1021/ac501591d

In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy. / Hosokawa, Masahito; Ando, Masahiro; Mukai, Shoichiro; Osada, Kyoko; Yoshino, Tomoko; Hamaguchi, Hiro O.; Tanaka, Tsuyoshi.

In: Analytical Chemistry, Vol. 86, No. 16, 19.08.2014, p. 8224-8230.

Research output: Contribution to journalArticle

Hosokawa, M, Ando, M, Mukai, S, Osada, K, Yoshino, T, Hamaguchi, HO & Tanaka, T 2014, 'In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy', Analytical Chemistry, vol. 86, no. 16, pp. 8224-8230. https://doi.org/10.1021/ac501591d
Hosokawa M, Ando M, Mukai S, Osada K, Yoshino T, Hamaguchi HO et al. In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy. Analytical Chemistry. 2014 Aug 19;86(16):8224-8230. https://doi.org/10.1021/ac501591d
Hosokawa, Masahito ; Ando, Masahiro ; Mukai, Shoichiro ; Osada, Kyoko ; Yoshino, Tomoko ; Hamaguchi, Hiro O. ; Tanaka, Tsuyoshi. / In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy. In: Analytical Chemistry. 2014 ; Vol. 86, No. 16. pp. 8224-8230.
@article{e9f2837beb814204b320a55fde280d0d,
title = "In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy",
abstract = "A straightforward in vivo monitoring technique for biomolecules would be an advantageous approach for understanding their spatiotemporal dynamics in living cells. However, the lack of adequate probes has hampered the quantitative determination of the chemical composition and metabolomics of cellular lipids at single-cell resolution. Here, we describe a method for the rapid, direct, and quantitative determination of lipid molecules from living cells using single-cell Raman imaging. In vivo localization of lipids in the form of triacylglycerol (TAG) within oleaginous microalga and their molecular compositions are monitored with high spatial resolution in a nondestructive and label-free manner. This method can provide quantitative and real-time information on compositions, chain lengths, and degree of unsaturation of fatty acids in living cells for improving the cultivating parameters or for determining the harvest timing during large-scale cultivations for microalgal lipid accumulation toward biodiesel production. Therefore, this technique is a potential tool for in vivo lipidomics for understanding the dynamics of lipid metabolisms in various organisms.",
author = "Masahito Hosokawa and Masahiro Ando and Shoichiro Mukai and Kyoko Osada and Tomoko Yoshino and Hamaguchi, {Hiro O.} and Tsuyoshi Tanaka",
year = "2014",
month = "8",
day = "19",
doi = "10.1021/ac501591d",
language = "English",
volume = "86",
pages = "8224--8230",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "16",

}

TY - JOUR

T1 - In vivo live cell imaging for the quantitative monitoring of lipids by using raman microspectroscopy

AU - Hosokawa, Masahito

AU - Ando, Masahiro

AU - Mukai, Shoichiro

AU - Osada, Kyoko

AU - Yoshino, Tomoko

AU - Hamaguchi, Hiro O.

AU - Tanaka, Tsuyoshi

PY - 2014/8/19

Y1 - 2014/8/19

N2 - A straightforward in vivo monitoring technique for biomolecules would be an advantageous approach for understanding their spatiotemporal dynamics in living cells. However, the lack of adequate probes has hampered the quantitative determination of the chemical composition and metabolomics of cellular lipids at single-cell resolution. Here, we describe a method for the rapid, direct, and quantitative determination of lipid molecules from living cells using single-cell Raman imaging. In vivo localization of lipids in the form of triacylglycerol (TAG) within oleaginous microalga and their molecular compositions are monitored with high spatial resolution in a nondestructive and label-free manner. This method can provide quantitative and real-time information on compositions, chain lengths, and degree of unsaturation of fatty acids in living cells for improving the cultivating parameters or for determining the harvest timing during large-scale cultivations for microalgal lipid accumulation toward biodiesel production. Therefore, this technique is a potential tool for in vivo lipidomics for understanding the dynamics of lipid metabolisms in various organisms.

AB - A straightforward in vivo monitoring technique for biomolecules would be an advantageous approach for understanding their spatiotemporal dynamics in living cells. However, the lack of adequate probes has hampered the quantitative determination of the chemical composition and metabolomics of cellular lipids at single-cell resolution. Here, we describe a method for the rapid, direct, and quantitative determination of lipid molecules from living cells using single-cell Raman imaging. In vivo localization of lipids in the form of triacylglycerol (TAG) within oleaginous microalga and their molecular compositions are monitored with high spatial resolution in a nondestructive and label-free manner. This method can provide quantitative and real-time information on compositions, chain lengths, and degree of unsaturation of fatty acids in living cells for improving the cultivating parameters or for determining the harvest timing during large-scale cultivations for microalgal lipid accumulation toward biodiesel production. Therefore, this technique is a potential tool for in vivo lipidomics for understanding the dynamics of lipid metabolisms in various organisms.

UR - http://www.scopus.com/inward/record.url?scp=84906222865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906222865&partnerID=8YFLogxK

U2 - 10.1021/ac501591d

DO - 10.1021/ac501591d

M3 - Article

AN - SCOPUS:84906222865

VL - 86

SP - 8224

EP - 8230

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 16

ER -