In vivo monitoring of peripheral circadian clocks in the mouse

Yu Tahara, Hiroaki Kuroda, Keisuke Saito, Yoshihiro Nakajima, Yuji Kubo, Nobuaki Ohnishi, Yasuhiro Seo, Makiko Otsuka, Yuta Fuse, Yuki Ohura, Takuya Komatsu, Youhei Moriya, Satoshi Okada, Naoki Furutani, Akiko Hirao, Kazumasa Horikawa, Takashi Kudo, Shigenobu Shibata

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Abstract

The mammalian circadian system is comprised of a central clock in the suprachiasmatic nucleus (SCN) and a network of peripheral oscillators located in all of the major organ systems [1, 2]. The SCN is traditionally thought to be positioned at the top of the hierarchy, with SCN lesions resulting in an arrhythmic organism [3]. However, recent work has demonstrated that the SCN and peripheral tissues generate independent circadian oscillations in Per1 clock gene expression in vitro [4]. In the present study, we sought to clarify the role of the SCN in the intact system by recording rhythms in clock gene expression in vivo. A practical imaging protocol was developed that enables us to measure circadian rhythms easily, noninvasively, and longitudinally in individual mice. Circadian oscillations were detected in the kidney, liver, and submandibular gland studied in about half of the SCN-lesioned, behaviorally arrhythmic mice. However, their amplitude was decreased in these organs. Free-running periods of peripheral clocks were identical to those of activity rhythms recorded before the SCN lesion. Thus, we can report for the first time that many of the fundamental properties of circadian oscillations in peripheral clocks in vivo are maintained in the absence of SCN control.

Original languageEnglish
Pages (from-to)1029-1034
Number of pages6
JournalCurrent Biology
Volume22
Issue number11
DOIs
Publication statusPublished - 2012 Jun 5

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tahara, Y., Kuroda, H., Saito, K., Nakajima, Y., Kubo, Y., Ohnishi, N., Seo, Y., Otsuka, M., Fuse, Y., Ohura, Y., Komatsu, T., Moriya, Y., Okada, S., Furutani, N., Hirao, A., Horikawa, K., Kudo, T., & Shibata, S. (2012). In vivo monitoring of peripheral circadian clocks in the mouse. Current Biology, 22(11), 1029-1034. https://doi.org/10.1016/j.cub.2012.04.009