In vivo release of prolactin in Rana ridibunda following an intravenous injection of thyrotropin-releasing hormone

E. R. Kühn, S. Kikuyama, K. Yamamoto, V. M. Darras

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    Abstract

    All experiments were performed on female Rana ridibunda during the months of April and May. Injections were made into the canulated vena abdominalis and blood samples were taken up to 8 hr thereafter and checked for prolactin concentration using a heterologous frog radioimmunoassay. An injection of 10 μg TRH (thyrotropin releasing hormone; pGlu-His-Pro NH2) will elevate control serum levels of 28.54 ± 5.40 ng/ml prolactin to 59.56 ± 8.78 ng/ml (n = 6) and this within half an hour. A smaller increase was present with 1 μg of TRH and none with 0.1 or 0.01 μg. Prolactin remains elevated up to 2 hr after the injection but reaches control values again after 4 hr. Two days after removal of the pars distalis serum levels have declined to 14.48 ± 1.67 ng/ml (n = 15) and no influence of TRH injections, tested at several doses, on this is seen. Previous thyroxine (T4) and tri-iodothyronine (T3) assays using the same blood have revealed that 1 and 10 μg TRH also increases T4 in a dose-related way after 1 hr, with a maximal increase at 2 hr, and T3 after 4 hr. It is concluded that TRH is able to induce the release of both prolactin and TSH from the hypophysis in the frog R. ridibunda as it does in mammals.

    Original languageEnglish
    Pages (from-to)86-89
    Number of pages4
    JournalGeneral and Comparative Endocrinology
    Volume60
    Issue number1
    DOIs
    Publication statusPublished - 1985

    Fingerprint

    Rana ridibunda
    thyrotropin-releasing hormone
    Thyrotropin-Releasing Hormone
    Rana
    prolactin
    intravenous injection
    Intravenous Injections
    Prolactin
    triiodothyronine
    injection
    Injections
    L-thyroxine
    blood serum
    Anura
    frogs
    pituitary gland
    blood
    Pituitary Gland
    thyroxine
    dosage

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    In vivo release of prolactin in Rana ridibunda following an intravenous injection of thyrotropin-releasing hormone. / Kühn, E. R.; Kikuyama, S.; Yamamoto, K.; Darras, V. M.

    In: General and Comparative Endocrinology, Vol. 60, No. 1, 1985, p. 86-89.

    Research output: Contribution to journalArticle

    Kühn, E. R. ; Kikuyama, S. ; Yamamoto, K. ; Darras, V. M. / In vivo release of prolactin in Rana ridibunda following an intravenous injection of thyrotropin-releasing hormone. In: General and Comparative Endocrinology. 1985 ; Vol. 60, No. 1. pp. 86-89.
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