Incorporation of DUF/FACT into chromatin enhances the accessibility of nucleosomal DNA

Hidetaka Seo, Koji Okuhara, Hitoshi Kurumizaka, Takatomi Yamada, Takehiko Shibata, Kunihiro Ohta, Tetsu Akiyama, Hiromu Murofushi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

DNA unwinding factor (DUF) was discovered as an essential DNA replication factor in Xenopus egg extracts. DUF consists of an HMG protein and a homolog of Cdc68p/Spt16p, and has the capability of unwinding dsDNA. Here we have examined the interaction of DUF with chromatin. DUF was incorporated into chromatin assembled from sperm heads and from plasmid DNA in egg extracts. It was revealed that the chromatin assembled in egg extracts immunodepleted of DUF is less sensitive to micrococcal nuclease (NNase) digestion than that assembled in control extracts, indicating that chromatin containing DUF has more decompact structure than that without DUF. Also we found that DUF has a high affinity for core histones in vitro. We suggest that the function of DUF may be to make the chromatin structure accessible to replication factors.

Original languageEnglish
Pages (from-to)8-13
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number1
DOIs
Publication statusPublished - 2003 Mar 28
Externally publishedYes

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Keywords

  • Chromatin
  • CP complex
  • DNA replication
  • DNA unwinding factor
  • FACT
  • Xenopus egg

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Seo, H., Okuhara, K., Kurumizaka, H., Yamada, T., Shibata, T., Ohta, K., Akiyama, T., & Murofushi, H. (2003). Incorporation of DUF/FACT into chromatin enhances the accessibility of nucleosomal DNA. Biochemical and Biophysical Research Communications, 303(1), 8-13. https://doi.org/10.1016/S0006-291X(03)00307-3