Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids

Kazuo Watanabe, Chikako Ishikawa, Hitomi Inoue, Deng Cenhua, Kazunaga Yazawa, Kiyosi Kondo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Incorporation of exogenous docosahexaenoic acid (DHA) into bacterial phospholipids was examined as a method for DHA-linked phospholipid production. The cultivation of 23 bacterial strains in medium with DHA showed that an eicosapentaenoic acid-producing bacterium Shewanella sp. strain SCRC-2738 (strain SCRC-2738), Bacillus subtilis W23, B. cereus, an Antarctic marine bacterium strain S-7 (strain S-7), photosynthesis bacterium (PSB) Rhodopseudomonas capsulatus utilized for the production of larval marine fish, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens and Escherichia coli K12 all incorporated DHA into their polar lipids. The polar lipids of the strain SCRC-2738, strain S-7, PSB and E. coli K12 were identified to be phospholipids. DHA was localized at the sn-2 position in the phospholipids of the four strains. Incorporation of exogenous DHA into their phospholipids produced an increase in saturated fatty acids and a decrease in monounsaturated fatty acids except E. coli K12. The strain SCRC-2738 incorporated the largest amount of DHA into their phospholipids among the tested bacterial strains in this study: DHA was 16% of the total fatty acids in the phosphatidylethanolamine (PE) and 29% in the phosphatidylglycerol (PG). In the PSB, incorporated DHA was 12% of the total fatty acids in the PE, 10% in the PG and phosophatidylcholine so that the PSB was nutritionally fortified.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume71
Issue number3
Publication statusPublished - 1994 Mar
Externally publishedYes

Fingerprint

Docosahexaenoic Acids
Phospholipids
docosahexaenoic acid
phospholipids
Acids
Bacteria
Photosynthesis
ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
Escherichia coli K12
Escherichia coli
photosynthesis
bacteria
Phosphatidylglycerols
Fatty Acids
phosphatidylglycerols
phosphatidylethanolamines
Fatty acids
Lipids
Shewanella
Monounsaturated fatty acids

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

Cite this

Watanabe, K., Ishikawa, C., Inoue, H., Cenhua, D., Yazawa, K., & Kondo, K. (1994). Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids. JAOCS, Journal of the American Oil Chemists' Society, 71(3), 325-330.

Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids. / Watanabe, Kazuo; Ishikawa, Chikako; Inoue, Hitomi; Cenhua, Deng; Yazawa, Kazunaga; Kondo, Kiyosi.

In: JAOCS, Journal of the American Oil Chemists' Society, Vol. 71, No. 3, 03.1994, p. 325-330.

Research output: Contribution to journalArticle

Watanabe, K, Ishikawa, C, Inoue, H, Cenhua, D, Yazawa, K & Kondo, K 1994, 'Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids', JAOCS, Journal of the American Oil Chemists' Society, vol. 71, no. 3, pp. 325-330.
Watanabe, Kazuo ; Ishikawa, Chikako ; Inoue, Hitomi ; Cenhua, Deng ; Yazawa, Kazunaga ; Kondo, Kiyosi. / Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids. In: JAOCS, Journal of the American Oil Chemists' Society. 1994 ; Vol. 71, No. 3. pp. 325-330.
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