Increased coccolith production by Emiliania huxleyi cultures enriched with dissolved inorganic carbon

H. Takano, R. Takei, E. Manabe, J. G. Burgess, M. Hirano, Tadashi Matsunaga

Research output: Contribution to journalArticle

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Abstract

Cells of Emiliania huxleyi grown on Eppley's medium enriched with dissolved inorganic carbon (DIC) developed multiple layers of coccoliths. The maximum diameter of cells grown in the presence of 13.2 mM DIC was 12.3 μm, whereas that of cells grown in the presence of 1.5 mM DIC was 8.0 μm. Although enrichment of Eppley's medium with DIC increased both coccolith production and cell growth, coccolith production was enhanced to a greater extent than cell growth. The enrichment of Eppley's medium with DIC was used to enhance production of coccolith particles by E. huxleyi. Repeated-batch culture, in which DIC, Ca2+, nitrate and phosphate concentrations in the medium were maintained by replacing the culture medium, was carried out in a closed photobioreactor. During repeated-batch culture, a maximum coccolith yield of 560 mg/l for 2 days and a maximum biomass yield of 810 mg/l for 2 days were achieved. Enrichment and maintenance of DIC is therefore an efficient method for the production of large quantities of coccoliths.

Original languageEnglish
Pages (from-to)460-465
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume43
Issue number3
DOIs
Publication statusPublished - 1995
Externally publishedYes

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Haptophyta
Carbon
Batch Cell Culture Techniques
Photobioreactors
Growth
Nitrates
Biomass
Culture Media
Phosphates
Maintenance

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Increased coccolith production by Emiliania huxleyi cultures enriched with dissolved inorganic carbon. / Takano, H.; Takei, R.; Manabe, E.; Burgess, J. G.; Hirano, M.; Matsunaga, Tadashi.

In: Applied Microbiology and Biotechnology, Vol. 43, No. 3, 1995, p. 460-465.

Research output: Contribution to journalArticle

Takano, H. ; Takei, R. ; Manabe, E. ; Burgess, J. G. ; Hirano, M. ; Matsunaga, Tadashi. / Increased coccolith production by Emiliania huxleyi cultures enriched with dissolved inorganic carbon. In: Applied Microbiology and Biotechnology. 1995 ; Vol. 43, No. 3. pp. 460-465.
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AU - Matsunaga, Tadashi

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AB - Cells of Emiliania huxleyi grown on Eppley's medium enriched with dissolved inorganic carbon (DIC) developed multiple layers of coccoliths. The maximum diameter of cells grown in the presence of 13.2 mM DIC was 12.3 μm, whereas that of cells grown in the presence of 1.5 mM DIC was 8.0 μm. Although enrichment of Eppley's medium with DIC increased both coccolith production and cell growth, coccolith production was enhanced to a greater extent than cell growth. The enrichment of Eppley's medium with DIC was used to enhance production of coccolith particles by E. huxleyi. Repeated-batch culture, in which DIC, Ca2+, nitrate and phosphate concentrations in the medium were maintained by replacing the culture medium, was carried out in a closed photobioreactor. During repeated-batch culture, a maximum coccolith yield of 560 mg/l for 2 days and a maximum biomass yield of 810 mg/l for 2 days were achieved. Enrichment and maintenance of DIC is therefore an efficient method for the production of large quantities of coccoliths.

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