Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset

Megumi Yamamoto, Motohiro Takeya, Hiroko Ikeshima-Kataoka, Masato Yasui, Yasuaki Kawasaki, Mitsuya Shiraishi, Eiji Majima, Seiji Shiraishi, Yasuhito Uezono, Masanori Sasaki, Komyo Eto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The relationship between methylmercury (MeHg) exposure and aquaporin (AQP) expression in the brain is currently unknown. To investigate this, we used a common marmoset model of acute MeHg exposure to examine AQP1, AQP4 and AQP11 gene expression. MeHg (1.5 mg Hg/kg/day p.o.) was given to three marmosets for 14 days, followed by 14 days without. All treated marmosets showed slight akinesia before sacrifice. In the frontal lobe, occipital lobe and cerebellum, total mercury concentrations following MeHg administration were 26.7, 31.4, and 22.6 μg/g, respectively. Slight apoptosis was observed in the occipital lobe. Immunohistochemistry showed increased expression of glial fibrillary acidic protein, its mRNA and Iba1 with MeHg, indicating that neuronal injury activated astrocytes and microglia. There was no significant difference between control and MeHg-administered groups in AQP1 protein or AQP11 mRNA in the frontal lobe, occipital lobe or cerebellum. The ratio of AQP4 mRNA expression in MeHg-administered marmosets to the mean AQR4 expression in the controls (n = 3) were 1.3, 1.5 and 1.2, 1.7, 1.9 and 1.5, and 1.5, 1.6 and 1.2 for the frontal lobe, occipital lobe and cerebellum, respectively. Western blotting showed significantly increased AQP4 protein in the occipital lobe and cerebellum with MeHg administration, but no obvious up-regulation in the frontal lobe. Immunofluorescence analysis with double staining revealed low AQP4 expression in the cell body of reactive astrocytes in the MeHg-administered group. These results indicate that AQP4 expression might be stimulated by MeHg exposure in astrocytes in the occipital lobe and cerebellum, suggesting a role for AQP4 in MeHg neurotoxicity via astrocyte dysfunction.

Original languageEnglish
Pages (from-to)749-763
Number of pages15
JournalJournal of Toxicological Sciences
Volume37
Issue number4
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Aquaporin 4
Callithrix
Occipital Lobe
Brain
Cerebellum
Frontal Lobe
Astrocytes
Messenger RNA
Aquaporin 1
Aquaporins
Glial Fibrillary Acidic Protein
Mercury
Gene expression
Cells
Microglia
Apoptosis
Fluorescent Antibody Technique
Up-Regulation
Western Blotting
Immunohistochemistry

Keywords

  • Aquaporin
  • Brain edema
  • Common marmoset
  • Methylmercury

ASJC Scopus subject areas

  • Toxicology

Cite this

Yamamoto, M., Takeya, M., Ikeshima-Kataoka, H., Yasui, M., Kawasaki, Y., Shiraishi, M., ... Eto, K. (2012). Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset. Journal of Toxicological Sciences, 37(4), 749-763. https://doi.org/10.2131/jts.37.749

Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset. / Yamamoto, Megumi; Takeya, Motohiro; Ikeshima-Kataoka, Hiroko; Yasui, Masato; Kawasaki, Yasuaki; Shiraishi, Mitsuya; Majima, Eiji; Shiraishi, Seiji; Uezono, Yasuhito; Sasaki, Masanori; Eto, Komyo.

In: Journal of Toxicological Sciences, Vol. 37, No. 4, 2012, p. 749-763.

Research output: Contribution to journalArticle

Yamamoto, M, Takeya, M, Ikeshima-Kataoka, H, Yasui, M, Kawasaki, Y, Shiraishi, M, Majima, E, Shiraishi, S, Uezono, Y, Sasaki, M & Eto, K 2012, 'Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset', Journal of Toxicological Sciences, vol. 37, no. 4, pp. 749-763. https://doi.org/10.2131/jts.37.749
Yamamoto, Megumi ; Takeya, Motohiro ; Ikeshima-Kataoka, Hiroko ; Yasui, Masato ; Kawasaki, Yasuaki ; Shiraishi, Mitsuya ; Majima, Eiji ; Shiraishi, Seiji ; Uezono, Yasuhito ; Sasaki, Masanori ; Eto, Komyo. / Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset. In: Journal of Toxicological Sciences. 2012 ; Vol. 37, No. 4. pp. 749-763.
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