Increased labelling of polyphosphoinositide in chemically transformed cell line C3H10T1/2 CL8

Y. Kubota, H. Inoue, T. Yoshioka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effect of malignant transformation of cells on phosphatidylinositol metabolism was investigated using C3H10T1/2 cells and its chemically transformed cell line, MCA CL-16 cells. We found that incorporation of [32P]P(i) into polyphosphoinositide was greatly increased in the transformed cells. A similar tendency was observed when myo-[2-3H]inositol was used as a labelling reagent. It is also observed that influx of labelled inorganic phosphate is enhanced 2-fold by the cell transformation. Therefore, promotion of polyphosphoinositide labelling in the transformed cell might be caused not only by the enhanced metabolism of phosphatidylinositol but also by the increased membrane permeability for radioactive labelling reagents.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume875
Issue number1
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

Phosphatidylinositol Phosphates
Transformed Cell Line
Labeling
Cells
Phosphatidylinositols
Metabolism
Inositol
Phosphates
Membranes
Permeability

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

Increased labelling of polyphosphoinositide in chemically transformed cell line C3H10T1/2 CL8. / Kubota, Y.; Inoue, H.; Yoshioka, T.

In: Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, Vol. 875, No. 1, 1986, p. 1-5.

Research output: Contribution to journalArticle

@article{e9ed5c67d4484c2f87ce9dc93c1da2a0,
title = "Increased labelling of polyphosphoinositide in chemically transformed cell line C3H10T1/2 CL8",
abstract = "The effect of malignant transformation of cells on phosphatidylinositol metabolism was investigated using C3H10T1/2 cells and its chemically transformed cell line, MCA CL-16 cells. We found that incorporation of [32P]P(i) into polyphosphoinositide was greatly increased in the transformed cells. A similar tendency was observed when myo-[2-3H]inositol was used as a labelling reagent. It is also observed that influx of labelled inorganic phosphate is enhanced 2-fold by the cell transformation. Therefore, promotion of polyphosphoinositide labelling in the transformed cell might be caused not only by the enhanced metabolism of phosphatidylinositol but also by the increased membrane permeability for radioactive labelling reagents.",
author = "Y. Kubota and H. Inoue and T. Yoshioka",
year = "1986",
language = "English",
volume = "875",
pages = "1--5",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Increased labelling of polyphosphoinositide in chemically transformed cell line C3H10T1/2 CL8

AU - Kubota, Y.

AU - Inoue, H.

AU - Yoshioka, T.

PY - 1986

Y1 - 1986

N2 - The effect of malignant transformation of cells on phosphatidylinositol metabolism was investigated using C3H10T1/2 cells and its chemically transformed cell line, MCA CL-16 cells. We found that incorporation of [32P]P(i) into polyphosphoinositide was greatly increased in the transformed cells. A similar tendency was observed when myo-[2-3H]inositol was used as a labelling reagent. It is also observed that influx of labelled inorganic phosphate is enhanced 2-fold by the cell transformation. Therefore, promotion of polyphosphoinositide labelling in the transformed cell might be caused not only by the enhanced metabolism of phosphatidylinositol but also by the increased membrane permeability for radioactive labelling reagents.

AB - The effect of malignant transformation of cells on phosphatidylinositol metabolism was investigated using C3H10T1/2 cells and its chemically transformed cell line, MCA CL-16 cells. We found that incorporation of [32P]P(i) into polyphosphoinositide was greatly increased in the transformed cells. A similar tendency was observed when myo-[2-3H]inositol was used as a labelling reagent. It is also observed that influx of labelled inorganic phosphate is enhanced 2-fold by the cell transformation. Therefore, promotion of polyphosphoinositide labelling in the transformed cell might be caused not only by the enhanced metabolism of phosphatidylinositol but also by the increased membrane permeability for radioactive labelling reagents.

UR - http://www.scopus.com/inward/record.url?scp=0022649199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022649199&partnerID=8YFLogxK

M3 - Article

C2 - 3000451

AN - SCOPUS:0022649199

VL - 875

SP - 1

EP - 5

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 1

ER -