Increased mitotic phosphorylation of histone H3 attributable to AIM-1/aurora-B overexpression contributes to chromosome number instability

Takahide Ota, Shiho Suto, Hiroshi Katayama, Zhen Bo Han, Fumio Suzuki, Masayo Maeda, Mikio Tanino, Yasuhiko Terada, Masaaki Tatsuka

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

Phosphorylation of histone H3 at Ser-10 is required for maintenance of proper chromosome dynamics during mitosis. AIM-1, a mammalian Ip11/aurora kinase involved in H3 phosphorylation, is transcriptionally overexpressed in many tumor cell lines. Increased expression of the AIM-1 gene has been observed in human colorectal tumors of advanced grade and stage. Here we report that forced exogenous overexpression of AIM-1 in Chinese hamster embryo cells causes increased mitotic Ser-10 phosphorylation with concomitant induction of lagging chromosomes during mitosis. Lagging chromosomes could also be induced by transfection with mutated histone H3 (S10E), which is thought to maintain Ser-10 in the phosphorylated state. In the present study, chromosome number instability and increased tumor invasiveness were noted in constitutively AIM-1-overexpressing cells in vivo. Increased mitotic Ser-10 phosphorylation was also observed in various colorectal tumor cells with high AIM-1 expression levels. These data suggest that increased H3 histone phosphorylation as a result of AIM-1 overexpression is a major precipitating factor of chromosome instability and, thus, may play a role in carcinogenesis.

Original languageEnglish
Pages (from-to)5168-5177
Number of pages10
JournalCancer Research
Volume62
Issue number18
Publication statusPublished - 2002 Sep 15
Externally publishedYes

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Chromosomal Instability
Histones
Phosphorylation
Chromosomes
Mitosis
Colorectal Neoplasms
Aurora Kinases
Precipitating Factors
Cricetulus
Tumor Cell Line
Transfection
Carcinogenesis
Embryonic Structures
Maintenance
Genes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ota, T., Suto, S., Katayama, H., Han, Z. B., Suzuki, F., Maeda, M., ... Tatsuka, M. (2002). Increased mitotic phosphorylation of histone H3 attributable to AIM-1/aurora-B overexpression contributes to chromosome number instability. Cancer Research, 62(18), 5168-5177.

Increased mitotic phosphorylation of histone H3 attributable to AIM-1/aurora-B overexpression contributes to chromosome number instability. / Ota, Takahide; Suto, Shiho; Katayama, Hiroshi; Han, Zhen Bo; Suzuki, Fumio; Maeda, Masayo; Tanino, Mikio; Terada, Yasuhiko; Tatsuka, Masaaki.

In: Cancer Research, Vol. 62, No. 18, 15.09.2002, p. 5168-5177.

Research output: Contribution to journalArticle

Ota, T, Suto, S, Katayama, H, Han, ZB, Suzuki, F, Maeda, M, Tanino, M, Terada, Y & Tatsuka, M 2002, 'Increased mitotic phosphorylation of histone H3 attributable to AIM-1/aurora-B overexpression contributes to chromosome number instability', Cancer Research, vol. 62, no. 18, pp. 5168-5177.
Ota, Takahide ; Suto, Shiho ; Katayama, Hiroshi ; Han, Zhen Bo ; Suzuki, Fumio ; Maeda, Masayo ; Tanino, Mikio ; Terada, Yasuhiko ; Tatsuka, Masaaki. / Increased mitotic phosphorylation of histone H3 attributable to AIM-1/aurora-B overexpression contributes to chromosome number instability. In: Cancer Research. 2002 ; Vol. 62, No. 18. pp. 5168-5177.
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