Increased resistance to nitric oxide cytotoxicity associated with differentiation of neuroblastoma-glioma hybrid (NG108-15) cells

Koichi Kawahara, Munetaka Saitoh, Takayuki Nakajima, Hideomi Sato, Motoki Tanaka, Takuro Tojima, Etsuro Ito

Research output: Contribution to journalReview article

9 Citations (Scopus)


Nitric oxide (NO), synthesized by the enzyme nitric oxide synthase (NOS), acts as an intercellular messenger associated with various physiological and pathological events. In this study, we investigated whether there exits a difference in the vulnerability to NO-induced cytotoxicity between undifferentiated and differentiated NG108-15 cells, and if so, the mechanisms responsible for the difference. Following a 7- to 8-day exposure to dibutyryl cAMP (dbcAMP), NG108-15 cells exhibited a neuron-like morphology associated with the expression of the neuronal protein, synaptophysin, and with increased NADPH-d activity. Neuron-like differentiated NG108-15 cells acquired resistance to exogenously applied NO. This increased resistance to NO toxicity in differentiated cells was almost completely cancelled out by inhibiting the activity of superoxide dismutase (SOD), but not by inhibiting the activity of NOS. The present study suggested that the activity of SOD increased in parallel with the activity of NOS associated with differentiation and was crucial for the acquired resistance to NO toxicity in differentiated cells.

Original languageEnglish
Pages (from-to)545-554
Number of pages10
JournalFree Radical Research
Issue number5
Publication statusPublished - 2002 May 1
Externally publishedYes



  • Cytotoxicity
  • Differentiation
  • NG108-15 cells
  • Nitric oxide
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry

Cite this