Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing aspergillus niger

Yuki Honda, Keiichi Kobayashi, Kotaro Kirimura

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    Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.

    Original languageEnglish
    Pages (from-to)1594-1596
    Number of pages3
    JournalBioscience, Biotechnology and Biochemistry
    Issue number8
    Publication statusPublished - 2011



    • Aspergillus niger
    • Citric acid production
    • Functional genomics
    • Gene-targeting
    • Ku80

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Analytical Chemistry
    • Organic Chemistry

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