Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing aspergillus niger

Yuki Honda, Keiichi Kobayashi, Kohtaro Kirimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.

Original languageEnglish
Pages (from-to)1594-1596
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number8
DOIs
Publication statusPublished - 2011 Aug 29

Keywords

  • Aspergillus niger
  • Citric acid production
  • Functional genomics
  • Gene-targeting
  • Ku80

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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