Independent stabilizations of polysomal Drg1/Dfrp1 complex and non-polysomal Drg2/Dfrp2 complex in mammalian cells

Kosuke Ishikawa, Taishin Akiyama, Koichi Ito, Kentaro Senba, Jun ichiro Inoue

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Various widely known GTPases are associated with diverse crucial cellular processes. However, the functional targets of the universally conserved homologous GTPases Drg1 and Drg2, constituting the DRG subfamily in eukaryotes, remain completely unknown despite their pleiotropic cell growth effects. Contrary to expectations of functional redundancy between Drg1 and Drg2 due to their high homology, the different binding proteins Dfrp1 and Dfrp2, respectively, have been previously identified. Here, we report the first systematic characterization of all these proteins in mammals by analyses in physiological conditions. Our findings are: (1) At least one of the components of the Drg1/Dfrp1 and the Drg2/Dfrp2 complexes is specifically and drastically stabilized by each unique complex formation; and (2) the Drg1/Dfrp1 complex cosediments with polysome, while neither Drg2 nor Dfrp2 is found in ribosomal fractions at all. These results suggest that the Drg1/Dfrp1 complex independently modulates a protein synthesis mechanism different from the Drg2/Dfrp2 complex in mammalian cells.

    Original languageEnglish
    Pages (from-to)552-556
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume390
    Issue number3
    DOIs
    Publication statusPublished - 2009 Dec 18

    Fingerprint

    GTP Phosphohydrolases
    Stabilization
    Cells
    Mammals
    Polyribosomes
    Diagnosis-Related Groups
    Cell growth
    Eukaryota
    Redundancy
    Carrier Proteins
    Proteins
    Growth

    Keywords

    • Noncanonical ribosome protein
    • Nonessential GTPase
    • Polysome

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Molecular Biology

    Cite this

    Independent stabilizations of polysomal Drg1/Dfrp1 complex and non-polysomal Drg2/Dfrp2 complex in mammalian cells. / Ishikawa, Kosuke; Akiyama, Taishin; Ito, Koichi; Senba, Kentaro; Inoue, Jun ichiro.

    In: Biochemical and Biophysical Research Communications, Vol. 390, No. 3, 18.12.2009, p. 552-556.

    Research output: Contribution to journalArticle

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