Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps

Tomoo Sawabe, Yohei Yamazaki, Pedro Milet Meirelles, Sayaka Mino, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Fabiano L. Thompson, Yuichi Sakai, Toko Sawabe

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Gut microbiome shapes various aspects of a host's physiology, but these functions in aquatic animal hosts have yet to be fully investigated. The sea cucumber Apostichopus japonicus Selenka is one such example. The large growth gap in their body size has delayed the development of intensive aquaculture, nevertheless the species is in urgent need of conservation. To understand possible contributions of the gut microbiome to its host's growth, individual fecal microbiome comparisons were performed. High-throughput 16S rRNA sequencing revealed significantly different microbiota in larger and smaller individuals; Rhodobacterales in particular was the most significantly abundant bacterial group in the larger specimens. Further shotgun metagenome of representative samples revealed a significant abundance of microbiome retaining polyhydroxybutyrate (PHB) metabolism genes in the largest individual. The PHB metabolism reads were potentially derived from Rhodobacterales. These results imply a possible link between microbial PHB producers and potential growth promotion in Deuterostomia marine invertebrates.

    Original languageEnglish
    Article number21631
    JournalScientific Reports
    Volume6
    DOIs
    Publication statusPublished - 2016 Feb 24

    Fingerprint

    Stichopus
    Microbiota
    Growth
    Sea Cucumbers
    Metagenome
    Aquaculture
    Firearms
    Body Size
    Invertebrates
    Genes
    Gastrointestinal Microbiome

    ASJC Scopus subject areas

    • General

    Cite this

    Sawabe, T., Yamazaki, Y., Meirelles, P. M., Mino, S., Suda, W., Oshima, K., ... Sawabe, T. (2016). Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps. Scientific Reports, 6, [21631]. https://doi.org/10.1038/srep21631

    Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps. / Sawabe, Tomoo; Yamazaki, Yohei; Meirelles, Pedro Milet; Mino, Sayaka; Suda, Wataru; Oshima, Kenshiro; Hattori, Masahira; Thompson, Fabiano L.; Sakai, Yuichi; Sawabe, Toko.

    In: Scientific Reports, Vol. 6, 21631, 24.02.2016.

    Research output: Contribution to journalArticle

    Sawabe, T, Yamazaki, Y, Meirelles, PM, Mino, S, Suda, W, Oshima, K, Hattori, M, Thompson, FL, Sakai, Y & Sawabe, T 2016, 'Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps', Scientific Reports, vol. 6, 21631. https://doi.org/10.1038/srep21631
    Sawabe, Tomoo ; Yamazaki, Yohei ; Meirelles, Pedro Milet ; Mino, Sayaka ; Suda, Wataru ; Oshima, Kenshiro ; Hattori, Masahira ; Thompson, Fabiano L. ; Sakai, Yuichi ; Sawabe, Toko. / Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps. In: Scientific Reports. 2016 ; Vol. 6.
    @article{5a1e6015c9f74738a08aa84c466724d4,
    title = "Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps",
    abstract = "Gut microbiome shapes various aspects of a host's physiology, but these functions in aquatic animal hosts have yet to be fully investigated. The sea cucumber Apostichopus japonicus Selenka is one such example. The large growth gap in their body size has delayed the development of intensive aquaculture, nevertheless the species is in urgent need of conservation. To understand possible contributions of the gut microbiome to its host's growth, individual fecal microbiome comparisons were performed. High-throughput 16S rRNA sequencing revealed significantly different microbiota in larger and smaller individuals; Rhodobacterales in particular was the most significantly abundant bacterial group in the larger specimens. Further shotgun metagenome of representative samples revealed a significant abundance of microbiome retaining polyhydroxybutyrate (PHB) metabolism genes in the largest individual. The PHB metabolism reads were potentially derived from Rhodobacterales. These results imply a possible link between microbial PHB producers and potential growth promotion in Deuterostomia marine invertebrates.",
    author = "Tomoo Sawabe and Yohei Yamazaki and Meirelles, {Pedro Milet} and Sayaka Mino and Wataru Suda and Kenshiro Oshima and Masahira Hattori and Thompson, {Fabiano L.} and Yuichi Sakai and Toko Sawabe",
    year = "2016",
    month = "2",
    day = "24",
    doi = "10.1038/srep21631",
    language = "English",
    volume = "6",
    journal = "Scientific Reports",
    issn = "2045-2322",
    publisher = "Nature Publishing Group",

    }

    TY - JOUR

    T1 - Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps

    AU - Sawabe, Tomoo

    AU - Yamazaki, Yohei

    AU - Meirelles, Pedro Milet

    AU - Mino, Sayaka

    AU - Suda, Wataru

    AU - Oshima, Kenshiro

    AU - Hattori, Masahira

    AU - Thompson, Fabiano L.

    AU - Sakai, Yuichi

    AU - Sawabe, Toko

    PY - 2016/2/24

    Y1 - 2016/2/24

    N2 - Gut microbiome shapes various aspects of a host's physiology, but these functions in aquatic animal hosts have yet to be fully investigated. The sea cucumber Apostichopus japonicus Selenka is one such example. The large growth gap in their body size has delayed the development of intensive aquaculture, nevertheless the species is in urgent need of conservation. To understand possible contributions of the gut microbiome to its host's growth, individual fecal microbiome comparisons were performed. High-throughput 16S rRNA sequencing revealed significantly different microbiota in larger and smaller individuals; Rhodobacterales in particular was the most significantly abundant bacterial group in the larger specimens. Further shotgun metagenome of representative samples revealed a significant abundance of microbiome retaining polyhydroxybutyrate (PHB) metabolism genes in the largest individual. The PHB metabolism reads were potentially derived from Rhodobacterales. These results imply a possible link between microbial PHB producers and potential growth promotion in Deuterostomia marine invertebrates.

    AB - Gut microbiome shapes various aspects of a host's physiology, but these functions in aquatic animal hosts have yet to be fully investigated. The sea cucumber Apostichopus japonicus Selenka is one such example. The large growth gap in their body size has delayed the development of intensive aquaculture, nevertheless the species is in urgent need of conservation. To understand possible contributions of the gut microbiome to its host's growth, individual fecal microbiome comparisons were performed. High-throughput 16S rRNA sequencing revealed significantly different microbiota in larger and smaller individuals; Rhodobacterales in particular was the most significantly abundant bacterial group in the larger specimens. Further shotgun metagenome of representative samples revealed a significant abundance of microbiome retaining polyhydroxybutyrate (PHB) metabolism genes in the largest individual. The PHB metabolism reads were potentially derived from Rhodobacterales. These results imply a possible link between microbial PHB producers and potential growth promotion in Deuterostomia marine invertebrates.

    UR - http://www.scopus.com/inward/record.url?scp=84959440949&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84959440949&partnerID=8YFLogxK

    U2 - 10.1038/srep21631

    DO - 10.1038/srep21631

    M3 - Article

    VL - 6

    JO - Scientific Reports

    JF - Scientific Reports

    SN - 2045-2322

    M1 - 21631

    ER -