Induced pluripotent stem cell differentiation under constant shear stress

N. K. Mohd Zin, K. Sakaguchi, Y. Haraguchi, T. Yagi, K. Matsuura, T. Shimizu, M. Umezu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.

Original languageEnglish
Title of host publication7th WACBE World Congress on Bioengineering, 2015
EditorsChwee Teck Lim, James Goh
PublisherSpringer Verlag
Pages7-10
Number of pages4
ISBN (Print)9783319194516
DOIs
Publication statusPublished - 2015
Event7th World Congress on Bioengineering, WACBE 2015 - Kent Ridge, Singapore
Duration: 2015 Jul 62015 Jul 8

Publication series

NameIFMBE Proceedings
Volume52
ISSN (Print)1680-0737

Other

Other7th World Congress on Bioengineering, WACBE 2015
Country/TerritorySingapore
CityKent Ridge
Period15/7/615/7/8

Keywords

  • Cell viability
  • IPSC
  • Shear stress

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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