### Abstract

Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.

Original language | English |
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Title of host publication | IFMBE Proceedings |

Publisher | Springer Verlag |

Pages | 7-10 |

Number of pages | 4 |

Volume | 52 |

ISBN (Print) | 9783319194516 |

DOIs | |

Publication status | Published - 2015 |

Event | 7th World Congress on Bioengineering, WACBE 2015 - Kent Ridge, Singapore Duration: 2015 Jul 6 → 2015 Jul 8 |

### Other

Other | 7th World Congress on Bioengineering, WACBE 2015 |
---|---|

Country | Singapore |

City | Kent Ridge |

Period | 15/7/6 → 15/7/8 |

### Fingerprint

### Keywords

- Cell viability
- IPSC
- Shear stress

### ASJC Scopus subject areas

- Biomedical Engineering
- Bioengineering

### Cite this

*IFMBE Proceedings*(Vol. 52, pp. 7-10). Springer Verlag. https://doi.org/10.1007/978-3-319-19452-3_3

**Induced pluripotent stem cell differentiation under constant shear stress.** / Mohd Zin, N. K.; Sakaguchi, Katsuhisa; Haraguchi, Y.; Yagi, Takanobu; Matsuura, K.; Shimizu, T.; Umezu, Mitsuo.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

*IFMBE Proceedings.*vol. 52, Springer Verlag, pp. 7-10, 7th World Congress on Bioengineering, WACBE 2015, Kent Ridge, Singapore, 15/7/6. https://doi.org/10.1007/978-3-319-19452-3_3

}

TY - GEN

T1 - Induced pluripotent stem cell differentiation under constant shear stress

AU - Mohd Zin, N. K.

AU - Sakaguchi, Katsuhisa

AU - Haraguchi, Y.

AU - Yagi, Takanobu

AU - Matsuura, K.

AU - Shimizu, T.

AU - Umezu, Mitsuo

PY - 2015

Y1 - 2015

N2 - Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.

AB - Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.

KW - Cell viability

KW - IPSC

KW - Shear stress

UR - http://www.scopus.com/inward/record.url?scp=84947437582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947437582&partnerID=8YFLogxK

U2 - 10.1007/978-3-319-19452-3_3

DO - 10.1007/978-3-319-19452-3_3

M3 - Conference contribution

SN - 9783319194516

VL - 52

SP - 7

EP - 10

BT - IFMBE Proceedings

PB - Springer Verlag

ER -