Induced pluripotent stem cell differentiation under constant shear stress

N. K. Mohd Zin, Katsuhisa Sakaguchi, Y. Haraguchi, Takanobu Yagi, K. Matsuura, T. Shimizu, Mitsuo Umezu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.

Original languageEnglish
Title of host publicationIFMBE Proceedings
PublisherSpringer Verlag
Pages7-10
Number of pages4
Volume52
ISBN (Print)9783319194516
DOIs
Publication statusPublished - 2015
Event7th World Congress on Bioengineering, WACBE 2015 - Kent Ridge, Singapore
Duration: 2015 Jul 62015 Jul 8

Other

Other7th World Congress on Bioengineering, WACBE 2015
CountrySingapore
CityKent Ridge
Period15/7/615/7/8

Fingerprint

Stem cells
Shear stress
Cells
Bioreactors
Laminar flow
Cytokines
Fluids

Keywords

  • Cell viability
  • IPSC
  • Shear stress

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Mohd Zin, N. K., Sakaguchi, K., Haraguchi, Y., Yagi, T., Matsuura, K., Shimizu, T., & Umezu, M. (2015). Induced pluripotent stem cell differentiation under constant shear stress. In IFMBE Proceedings (Vol. 52, pp. 7-10). Springer Verlag. https://doi.org/10.1007/978-3-319-19452-3_3

Induced pluripotent stem cell differentiation under constant shear stress. / Mohd Zin, N. K.; Sakaguchi, Katsuhisa; Haraguchi, Y.; Yagi, Takanobu; Matsuura, K.; Shimizu, T.; Umezu, Mitsuo.

IFMBE Proceedings. Vol. 52 Springer Verlag, 2015. p. 7-10.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mohd Zin, NK, Sakaguchi, K, Haraguchi, Y, Yagi, T, Matsuura, K, Shimizu, T & Umezu, M 2015, Induced pluripotent stem cell differentiation under constant shear stress. in IFMBE Proceedings. vol. 52, Springer Verlag, pp. 7-10, 7th World Congress on Bioengineering, WACBE 2015, Kent Ridge, Singapore, 15/7/6. https://doi.org/10.1007/978-3-319-19452-3_3
Mohd Zin NK, Sakaguchi K, Haraguchi Y, Yagi T, Matsuura K, Shimizu T et al. Induced pluripotent stem cell differentiation under constant shear stress. In IFMBE Proceedings. Vol. 52. Springer Verlag. 2015. p. 7-10 https://doi.org/10.1007/978-3-319-19452-3_3
Mohd Zin, N. K. ; Sakaguchi, Katsuhisa ; Haraguchi, Y. ; Yagi, Takanobu ; Matsuura, K. ; Shimizu, T. ; Umezu, Mitsuo. / Induced pluripotent stem cell differentiation under constant shear stress. IFMBE Proceedings. Vol. 52 Springer Verlag, 2015. pp. 7-10
@inproceedings{cfe8a4abec424c06a0a2f5789d1194b5,
title = "Induced pluripotent stem cell differentiation under constant shear stress",
abstract = "Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.",
keywords = "Cell viability, IPSC, Shear stress",
author = "{Mohd Zin}, {N. K.} and Katsuhisa Sakaguchi and Y. Haraguchi and Takanobu Yagi and K. Matsuura and T. Shimizu and Mitsuo Umezu",
year = "2015",
doi = "10.1007/978-3-319-19452-3_3",
language = "English",
isbn = "9783319194516",
volume = "52",
pages = "7--10",
booktitle = "IFMBE Proceedings",
publisher = "Springer Verlag",

}

TY - GEN

T1 - Induced pluripotent stem cell differentiation under constant shear stress

AU - Mohd Zin, N. K.

AU - Sakaguchi, Katsuhisa

AU - Haraguchi, Y.

AU - Yagi, Takanobu

AU - Matsuura, K.

AU - Shimizu, T.

AU - Umezu, Mitsuo

PY - 2015

Y1 - 2015

N2 - Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.

AB - Mechanotransduction in in-vitro studies has yet to be done extensively using human induced pluripotent (hiPS) cells. These external mechanical factors are important controlling factor for cell differentiation at a cellular level particularly in cardiomyocytes cells. Cells in a living body are being exposed to many kind of stresses; shear stress in particular modulates cellular function in a living body. Similarly, we are trying to understand the link between the shear stress in the bioreactor and the differentiation effects it has on iPS cells. In this study, we investigate the difference of strictly uniform laminar shear stress with a non-uniform laminar flow and its effects on the cell survival rate and differentiation. Additionally, the elimination of biochemical factors were done to satisfy the need of demand for clinically usable cells for the near future use. By eliminating cytokine induced differentiation. In addition, information pertaining to relationship between fluid shear stress, cellular deformations, cell differentiation and cell survival rate could provide a more optimised condition for cultivation specific type of cells within a shorter time period.

KW - Cell viability

KW - IPSC

KW - Shear stress

UR - http://www.scopus.com/inward/record.url?scp=84947437582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947437582&partnerID=8YFLogxK

U2 - 10.1007/978-3-319-19452-3_3

DO - 10.1007/978-3-319-19452-3_3

M3 - Conference contribution

AN - SCOPUS:84947437582

SN - 9783319194516

VL - 52

SP - 7

EP - 10

BT - IFMBE Proceedings

PB - Springer Verlag

ER -