Induction of albumin expression in HepG2 cells using immobilized simplified recombinant fibronectin protein

Yuuki Nishida, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Optimization of the extracellular environment is very important for hepatocyte function in vitro. We expressed new chimeric proteins of the collagen-binding domain (CBD) with cell attachment site (CAS) of fibronectin to enhance hepatocyte function, and the CBD-CAS proteins were immobilized on collagen-coated plates. We hypothesized that the high density of CAS would increase activity of the integrin-dependent intracellular signaling pathway, thus inducing hepatocyte function. Expression of albumin in the human hepatocyte cell line HepG2 was assessed on CBD-CAS-immobilized dishes. The results indicated that the CBD-CAS-immobilized plates induced albumin expression. Immobilized CBD-CAS induced activation of focal adhesion kinase and integrin-ligand clustering on the cell membrane. These results suggest that immobilized CBD-CAS improves the function of HepG2 cells. This system could therefore be applied to drug metabolism assay in the development of new drugs.

    Original languageEnglish
    Pages (from-to)400-407
    Number of pages8
    JournalIn Vitro Cellular and Developmental Biology - Animal
    Volume49
    Issue number6
    DOIs
    Publication statusPublished - 2013 Jun

    Fingerprint

    Hep G2 Cells
    Fibronectins
    Recombinant Proteins
    Albumins
    Collagen
    Hepatocytes
    Integrins
    Immobilized Proteins
    Focal Adhesion Protein-Tyrosine Kinases
    Protein Binding
    Pharmaceutical Preparations
    Cluster Analysis
    Cell Count
    Cell Membrane
    Ligands
    Cell Line

    Keywords

    • Fibronectin
    • Hepatocyte
    • Recombinant protein

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

    Cite this

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    title = "Induction of albumin expression in HepG2 cells using immobilized simplified recombinant fibronectin protein",
    abstract = "Optimization of the extracellular environment is very important for hepatocyte function in vitro. We expressed new chimeric proteins of the collagen-binding domain (CBD) with cell attachment site (CAS) of fibronectin to enhance hepatocyte function, and the CBD-CAS proteins were immobilized on collagen-coated plates. We hypothesized that the high density of CAS would increase activity of the integrin-dependent intracellular signaling pathway, thus inducing hepatocyte function. Expression of albumin in the human hepatocyte cell line HepG2 was assessed on CBD-CAS-immobilized dishes. The results indicated that the CBD-CAS-immobilized plates induced albumin expression. Immobilized CBD-CAS induced activation of focal adhesion kinase and integrin-ligand clustering on the cell membrane. These results suggest that immobilized CBD-CAS improves the function of HepG2 cells. This system could therefore be applied to drug metabolism assay in the development of new drugs.",
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    author = "Yuuki Nishida and Akiyoshi Taniguchi",
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    TY - JOUR

    T1 - Induction of albumin expression in HepG2 cells using immobilized simplified recombinant fibronectin protein

    AU - Nishida, Yuuki

    AU - Taniguchi, Akiyoshi

    PY - 2013/6

    Y1 - 2013/6

    N2 - Optimization of the extracellular environment is very important for hepatocyte function in vitro. We expressed new chimeric proteins of the collagen-binding domain (CBD) with cell attachment site (CAS) of fibronectin to enhance hepatocyte function, and the CBD-CAS proteins were immobilized on collagen-coated plates. We hypothesized that the high density of CAS would increase activity of the integrin-dependent intracellular signaling pathway, thus inducing hepatocyte function. Expression of albumin in the human hepatocyte cell line HepG2 was assessed on CBD-CAS-immobilized dishes. The results indicated that the CBD-CAS-immobilized plates induced albumin expression. Immobilized CBD-CAS induced activation of focal adhesion kinase and integrin-ligand clustering on the cell membrane. These results suggest that immobilized CBD-CAS improves the function of HepG2 cells. This system could therefore be applied to drug metabolism assay in the development of new drugs.

    AB - Optimization of the extracellular environment is very important for hepatocyte function in vitro. We expressed new chimeric proteins of the collagen-binding domain (CBD) with cell attachment site (CAS) of fibronectin to enhance hepatocyte function, and the CBD-CAS proteins were immobilized on collagen-coated plates. We hypothesized that the high density of CAS would increase activity of the integrin-dependent intracellular signaling pathway, thus inducing hepatocyte function. Expression of albumin in the human hepatocyte cell line HepG2 was assessed on CBD-CAS-immobilized dishes. The results indicated that the CBD-CAS-immobilized plates induced albumin expression. Immobilized CBD-CAS induced activation of focal adhesion kinase and integrin-ligand clustering on the cell membrane. These results suggest that immobilized CBD-CAS improves the function of HepG2 cells. This system could therefore be applied to drug metabolism assay in the development of new drugs.

    KW - Fibronectin

    KW - Hepatocyte

    KW - Recombinant protein

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