Induction of albumin expression in HepG2 cells using immobilized simplified recombinant fibronectin protein

Yuuki Nishida, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Optimization of the extracellular environment is very important for hepatocyte function in vitro. We expressed new chimeric proteins of the collagen-binding domain (CBD) with cell attachment site (CAS) of fibronectin to enhance hepatocyte function, and the CBD-CAS proteins were immobilized on collagen-coated plates. We hypothesized that the high density of CAS would increase activity of the integrin-dependent intracellular signaling pathway, thus inducing hepatocyte function. Expression of albumin in the human hepatocyte cell line HepG2 was assessed on CBD-CAS-immobilized dishes. The results indicated that the CBD-CAS-immobilized plates induced albumin expression. Immobilized CBD-CAS induced activation of focal adhesion kinase and integrin-ligand clustering on the cell membrane. These results suggest that immobilized CBD-CAS improves the function of HepG2 cells. This system could therefore be applied to drug metabolism assay in the development of new drugs.

Original languageEnglish
Pages (from-to)400-407
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume49
Issue number6
DOIs
Publication statusPublished - 2013 Jun 1

Keywords

  • Fibronectin
  • Hepatocyte
  • Recombinant protein

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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