Induction of hepatocyte functional protein expression by submicron/nano-patterning substrates to mimic in vivo structures

Shimaa A. Abdellatef, Akihiko Ohi, Toshihide Nabatame, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    To investigate the influence of bio-inspired metallic superficial topography on the cellular behaviour of a hepatocyte cell line, TiO2 nanopatterns with diversified shapes and heterotropic lateral dimensions were fabricated using electron beam lithography and atomic layer deposition. The dimensional uniformity and shape diversity of the nanopatterns were confirmed using scanning electron microscopy and atomic force microscopy. These topographical nanocues provide good tools for controlling and regulating multiple hepatocellular functions. The expressions of functional proteins such as albumin, transferrin and cytochrome P450 were tested as functional markers. In addition, the change in cellular orientation, cell alignment and native extracellular matrix (ECM) assembly induced by these well-defined nanotopographies were observed. Twelve hours after cell seeding, TiO2 nanogratings with a lateral dimension of 240 nm showed a higher degree of functional protein expression compared to other nanotopographical substrates and a flat surface. These findings suggest that the TiO2 surface resembles a hierarchically-extended collagen nanofibrillar surface and could be recognized by hepatocytes, allowing the proper cytoskeletal orientation and cellular integrity. This TiO2 nanopattern with a specific shape and dimension (240 nm) might therefore emulate ECM biophysical cues, and the intrinsic topography of TiO2 surfaces might evoke enhanced cellular responses. These unique surfaces could be further exploited for tissue engineering and bioreactor technology.

    Original languageEnglish
    Pages (from-to)330-338
    Number of pages9
    JournalBiomaterials Science
    Volume2
    Issue number3
    DOIs
    Publication statusPublished - 2014 Mar

    Fingerprint

    Proteins
    Substrates
    Topography
    Atomic layer deposition
    Electron beam lithography
    Transferrin
    Bioreactors
    Tissue engineering
    Collagen
    Cytochrome P-450 Enzyme System
    Albumins
    Atomic force microscopy
    Cells
    Hepatocytes
    Scanning electron microscopy

    ASJC Scopus subject areas

    • Materials Science(all)
    • Biomedical Engineering

    Cite this

    Induction of hepatocyte functional protein expression by submicron/nano-patterning substrates to mimic in vivo structures. / Abdellatef, Shimaa A.; Ohi, Akihiko; Nabatame, Toshihide; Taniguchi, Akiyoshi.

    In: Biomaterials Science, Vol. 2, No. 3, 03.2014, p. 330-338.

    Research output: Contribution to journalArticle

    @article{4348350ae5c348d6bad22cf6cc1402b9,
    title = "Induction of hepatocyte functional protein expression by submicron/nano-patterning substrates to mimic in vivo structures",
    abstract = "To investigate the influence of bio-inspired metallic superficial topography on the cellular behaviour of a hepatocyte cell line, TiO2 nanopatterns with diversified shapes and heterotropic lateral dimensions were fabricated using electron beam lithography and atomic layer deposition. The dimensional uniformity and shape diversity of the nanopatterns were confirmed using scanning electron microscopy and atomic force microscopy. These topographical nanocues provide good tools for controlling and regulating multiple hepatocellular functions. The expressions of functional proteins such as albumin, transferrin and cytochrome P450 were tested as functional markers. In addition, the change in cellular orientation, cell alignment and native extracellular matrix (ECM) assembly induced by these well-defined nanotopographies were observed. Twelve hours after cell seeding, TiO2 nanogratings with a lateral dimension of 240 nm showed a higher degree of functional protein expression compared to other nanotopographical substrates and a flat surface. These findings suggest that the TiO2 surface resembles a hierarchically-extended collagen nanofibrillar surface and could be recognized by hepatocytes, allowing the proper cytoskeletal orientation and cellular integrity. This TiO2 nanopattern with a specific shape and dimension (240 nm) might therefore emulate ECM biophysical cues, and the intrinsic topography of TiO2 surfaces might evoke enhanced cellular responses. These unique surfaces could be further exploited for tissue engineering and bioreactor technology.",
    author = "Abdellatef, {Shimaa A.} and Akihiko Ohi and Toshihide Nabatame and Akiyoshi Taniguchi",
    year = "2014",
    month = "3",
    doi = "10.1039/c3bm60191a",
    language = "English",
    volume = "2",
    pages = "330--338",
    journal = "Biomaterials Science",
    issn = "2047-4830",
    publisher = "Royal Society of Chemistry",
    number = "3",

    }

    TY - JOUR

    T1 - Induction of hepatocyte functional protein expression by submicron/nano-patterning substrates to mimic in vivo structures

    AU - Abdellatef, Shimaa A.

    AU - Ohi, Akihiko

    AU - Nabatame, Toshihide

    AU - Taniguchi, Akiyoshi

    PY - 2014/3

    Y1 - 2014/3

    N2 - To investigate the influence of bio-inspired metallic superficial topography on the cellular behaviour of a hepatocyte cell line, TiO2 nanopatterns with diversified shapes and heterotropic lateral dimensions were fabricated using electron beam lithography and atomic layer deposition. The dimensional uniformity and shape diversity of the nanopatterns were confirmed using scanning electron microscopy and atomic force microscopy. These topographical nanocues provide good tools for controlling and regulating multiple hepatocellular functions. The expressions of functional proteins such as albumin, transferrin and cytochrome P450 were tested as functional markers. In addition, the change in cellular orientation, cell alignment and native extracellular matrix (ECM) assembly induced by these well-defined nanotopographies were observed. Twelve hours after cell seeding, TiO2 nanogratings with a lateral dimension of 240 nm showed a higher degree of functional protein expression compared to other nanotopographical substrates and a flat surface. These findings suggest that the TiO2 surface resembles a hierarchically-extended collagen nanofibrillar surface and could be recognized by hepatocytes, allowing the proper cytoskeletal orientation and cellular integrity. This TiO2 nanopattern with a specific shape and dimension (240 nm) might therefore emulate ECM biophysical cues, and the intrinsic topography of TiO2 surfaces might evoke enhanced cellular responses. These unique surfaces could be further exploited for tissue engineering and bioreactor technology.

    AB - To investigate the influence of bio-inspired metallic superficial topography on the cellular behaviour of a hepatocyte cell line, TiO2 nanopatterns with diversified shapes and heterotropic lateral dimensions were fabricated using electron beam lithography and atomic layer deposition. The dimensional uniformity and shape diversity of the nanopatterns were confirmed using scanning electron microscopy and atomic force microscopy. These topographical nanocues provide good tools for controlling and regulating multiple hepatocellular functions. The expressions of functional proteins such as albumin, transferrin and cytochrome P450 were tested as functional markers. In addition, the change in cellular orientation, cell alignment and native extracellular matrix (ECM) assembly induced by these well-defined nanotopographies were observed. Twelve hours after cell seeding, TiO2 nanogratings with a lateral dimension of 240 nm showed a higher degree of functional protein expression compared to other nanotopographical substrates and a flat surface. These findings suggest that the TiO2 surface resembles a hierarchically-extended collagen nanofibrillar surface and could be recognized by hepatocytes, allowing the proper cytoskeletal orientation and cellular integrity. This TiO2 nanopattern with a specific shape and dimension (240 nm) might therefore emulate ECM biophysical cues, and the intrinsic topography of TiO2 surfaces might evoke enhanced cellular responses. These unique surfaces could be further exploited for tissue engineering and bioreactor technology.

    UR - http://www.scopus.com/inward/record.url?scp=84893340405&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84893340405&partnerID=8YFLogxK

    U2 - 10.1039/c3bm60191a

    DO - 10.1039/c3bm60191a

    M3 - Article

    VL - 2

    SP - 330

    EP - 338

    JO - Biomaterials Science

    JF - Biomaterials Science

    SN - 2047-4830

    IS - 3

    ER -