Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor

M. Sakakibara, D. L. Alkon, T. Kouchi, H. Inoue, T. Yoshioka

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) was obtained. Neomycin and spermine, which inhibit PIP2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP3), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.

Original languageEnglish
Pages (from-to)299-306
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number1
DOIs
Publication statusPublished - 1994 Jul 15
Externally publishedYes

Fingerprint

Hermissenda
Phosphatidylinositol Phosphates
Phosphatidylinositols
R 59022
Hydrolysis
Guanosine 5'-O-(3-Thiotriphosphate)
Light
Photoreceptor Cells
Injections
Inositol 1,4,5-Trisphosphate
Neomycin
Spermine
Guanosine
Diphosphates

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor. / Sakakibara, M.; Alkon, D. L.; Kouchi, T.; Inoue, H.; Yoshioka, T.

In: Biochemical and Biophysical Research Communications, Vol. 202, No. 1, 15.07.1994, p. 299-306.

Research output: Contribution to journalArticle

Sakakibara, M. ; Alkon, D. L. ; Kouchi, T. ; Inoue, H. ; Yoshioka, T. / Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 202, No. 1. pp. 299-306.
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