Abstract
Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) was obtained. Neomycin and spermine, which inhibit PIP2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP3), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.
| Original language | English |
|---|---|
| Pages (from-to) | 299-306 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 202 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1994 Jul 15 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor. / Sakakibara, M.; Alkon, D. L.; Kouchi, T.; Inoue, H.; Yoshioka, T.
In: Biochemical and Biophysical Research Communications, Vol. 202, No. 1, 15.07.1994, p. 299-306.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor
AU - Sakakibara, M.
AU - Alkon, D. L.
AU - Kouchi, T.
AU - Inoue, H.
AU - Yoshioka, T.
PY - 1994/7/15
Y1 - 1994/7/15
N2 - Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) was obtained. Neomycin and spermine, which inhibit PIP2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP3), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.
AB - Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) was obtained. Neomycin and spermine, which inhibit PIP2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP3), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.
UR - http://www.scopus.com/inward/record.url?scp=0028168120&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028168120&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.1927
DO - 10.1006/bbrc.1994.1927
M3 - Article
VL - 202
SP - 299
EP - 306
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -