Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor

M. Sakakibara; D. L. Alkon; T. Kouchi; H. Inoue; T. Yoshioka

doi:10.1006/bbrc.1994.1927

Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor

M. Sakakibara, D. L. Alkon, T. Kouchi, H. Inoue, T. Yoshioka

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP₂) was obtained. Neomycin and spermine, which inhibit PIP₂ breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP₃), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na⁺ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP₂ induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.

Original language	English
Pages (from-to)	299-306
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	202
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1994.1927
Publication status	Published - 1994 Jul 15
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry

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title = "Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor",

abstract = "Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) was obtained. Neomycin and spermine, which inhibit PIP2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP3), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.",

author = "M. Sakakibara and Alkon, {D. L.} and T. Kouchi and H. Inoue and T. Yoshioka",

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AU - Sakakibara, M.

AU - Alkon, D. L.

AU - Kouchi, T.

AU - Inoue, H.

AU - Yoshioka, T.

PY - 1994/7/15

Y1 - 1994/7/15

N2 - Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) was obtained. Neomycin and spermine, which inhibit PIP2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP3), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.

AB - Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) was obtained. Neomycin and spermine, which inhibit PIP2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP3), guanosine 5′-(3-O-thio)triphosphate (GTPγS), guanosine 5′-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.

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