Influence of ammonium chloride on growth and fatty acid production by spirulina platensis

Eichi Manabe, Morio Hirano, Hiroyuki Takano, Naoko Ishikawa-Doi, Koji Sode, Tadashi Matsunaga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Changes in growth and fatty acid content of Spirulina platensis were examined after transferring cells into media containing various concentrations of ammonium chloride. Photosynthetic O2 evolution rate decreased with increasing ammonium chloride concentration. Therefore, the algal growth was interrupted by ammonium chloride addition. On the other hand, total fatty acid content markedly increased after addition of ammonium chloride to a concentration of 15-50 mM and was maximized 40-48 h after addition of 25 mM ammonium chloride. The increases in palmitic and oleic acid content were especially remarkable. However, this began to decrease 48 h after the addition of 25 mM of ammonium chloride. Also, γ-linolenic acid content increased continuously during a 72-h incubation. As a result, Spirulina platensis cells containing about 2% γ-linolenic acid were obtained by ammonium chloride treatment, representing an increase of 1.5-2-fold compared to untreated cells.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalApplied Biochemistry and Biotechnology
Volume34-35
Issue number1
DOIs
Publication statusPublished - 1992 Mar
Externally publishedYes

Fingerprint

Spirulina
Ammonium Chloride
Fatty acids
Fatty Acids
Palmitic acid
Acids
Oleic acid
Growth
alpha-Linolenic Acid
Palmitic Acid
Oleic Acid

Keywords

  • γ-linolenic acid
  • ammonium chloride
  • blue-green alga
  • fatty acid
  • Spirulina platensis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Molecular Biology
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Influence of ammonium chloride on growth and fatty acid production by spirulina platensis. / Manabe, Eichi; Hirano, Morio; Takano, Hiroyuki; Ishikawa-Doi, Naoko; Sode, Koji; Matsunaga, Tadashi.

In: Applied Biochemistry and Biotechnology, Vol. 34-35, No. 1, 03.1992, p. 273-281.

Research output: Contribution to journalArticle

Manabe, Eichi ; Hirano, Morio ; Takano, Hiroyuki ; Ishikawa-Doi, Naoko ; Sode, Koji ; Matsunaga, Tadashi. / Influence of ammonium chloride on growth and fatty acid production by spirulina platensis. In: Applied Biochemistry and Biotechnology. 1992 ; Vol. 34-35, No. 1. pp. 273-281.
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